Sentences with phrase «then cultured the cells»

«Then we cultured the cells in the presence of various drugs and screened for spontaneous drug - resistant mutants to isolate clones useful for symbiosis experiments.»

The team then cultured the cells for several months in a bath of growth - promoting proteins, trying to get the cells to reverse course and become more like stem cells.

The team collected two small skin samples from each, and then cultured the cells in the lab and inserted the gene that makes fireflies glow into the gene that regulates the cells» clock.

Not exact matches

[Perhaps the concept of «church - court» has moved to the openness of the Internet, and we all are now part of the greater «church court»... but then again, I can only wonder how to implement the third step within a global culture interconnected by the Internet, cell phones, tweets, and videos....

If Christians believe that the church and the worship it offers to God ought in some ways to counter the norms and practices of the surrounding culture, then what does it mean that after spending so much of our time each week in front of computer monitors, cell phones, and sports bar TVs, we come to church on Sunday and happily position ourselves in front of the biggest screen of all?

In giant vats, muscle and fat cells will be cultured then made into so - called «clean meat», without, if the technology progresses, a single animal having to die.

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

The next step, Silber says, will be harvesting stem cells from around a «spermless» man's testicles, introducing them into cultures where the cells multiply, and then reimplanting those cells in the man's testicles, which then nurture them into fully mature sperm - producers.

Setton's lab exposed the hiPSCs to a variety of different growth factors and culture media to coax them into first developing markers for, and then fully forming into, notochord cells.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

The researchers then examined a number of DNA variants in the protein production - control region of the bellwether gene in cell cultures.

The team pumped around 50 million rat liver cells into each of five bare scaffolds, then incubated the organs in culture for two weeks.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

They then used fluorescent antibodies that bind specifically to the protein to trace the virus's interactions with various kinds of cultured cells.

«It might be possible to grow these cells in culture then bank them so that if or when the mother develops some disease later in life, such as diabetes, her cells may be defrosted and differentiated into pancreatic beta cells,» says Lyle Armstrong of Newcastle University, UK, although he too, cautions that more tests are needed to determine exactly what these cells are.

Under experimental conditions, the researchers then demonstrated that elevated levels of elastase dramatically reduce the survival and operation of muscle stem cells in culture.

The cells were put into a special culture and then suspended in a gel and stimulated by nutrients, all geared to turn them into neurons like those found in the cortex.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

This was observed in human ovarian cancer cells grown in culture, and then in mouse models of the disease.

Hayashi then drew on other research showing that one key regulatory molecule (activin A) and a growth factor (basic fibroblast growth factor) could convert cultured early embryonic stem cells into cells akin to epiblasts.

Then they culture heart cells near the junction of the head and the tail.

Working with researchers at Johns Hopkins University in Baltimore and the University of California, San Francisco, Hynes and Rosenthal then tried adding different concentrations of Sonic hedgehog to cultured brain cells.

To make the vaccine, cancer cells are harvested from a tumor after surgery and stripped of their proteins; then those proteins are cultured with dendritic cells, a subclass of white blood cells, drawn from the patient's blood.

Dendritic cells are too large to pass through the blood - brain barrier, which is one reason they have to be cultured with brain tumor cells outside the body, then injected back into the patient's skin as a vaccine.

But then he observed the same halt in cell division in other cultures with different fetal tissues.

Hsiao and colleagues carefully gathered skin samples from donors, and then grew the skin cells in culture before converting them into iPS cells using the methods created by Yamanaka.

The researchers isolated the milk ducts and purified the breast - tissue cells to create a cell culture, which was then tested for different gene regulation profiles.

Previously, research groups had to get approval for each project involving human ES cells from their own Institutional Review Board (IRB) and then from a national committee under the Ministry of Education, Culture, Science, Sports, and Technology.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

«People have taken cells and then transformed them in culture and stuck them into animals,» Pollard says.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

Adoptive cell transfer procedures are mimicking exactly this process in a culture dish by taking T cells from patients, multiplying them, sometimes genetically modifying them, and then returning them to patients so that they can, for example, locate and kill cancer cells.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

To survey exactly which bacteria induced TH1 cells in the gut of the TH1 induced mice the researchers isolated and cultured the microbes from the feces, and then separately inoculated each microbe to germ - free mice.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

They then added eight or nine cell types back onto the matrix and cultured them for eight days.

Then, to test the notion that a CAG repeat could cause this nuclear clumping, the team inserted a shortened MJD gene containing a long CAG repeat (78 CAGs) into cultured cells and watched the protein product migrate into the nucleus, aggregate there, and recruit the normal MJD protein into a tangle.

Wagers, then a postdoctoral fellow in the Stanford University lab of stem cell biologist Irving Weissman, spent a week in Verfaillie's lab in 2002 trying to learn to culture MAPCs.

To do this, they incorporated the gene sequence of each receptor into cultured cells and then probed the cells to determine if they were activated by one or more of 25 different bitter - tasting chemicals.

Then, by placing surface proteins of each HCV virus onto these pseudoviruses, the researchers were able to efficiently infect human cells with the HCV strains in tissue culture.

Critically, despite the detection of enhanced Zika infection in cell culture models using antibodies from macaques previously exposed to either yellow fever or dengue and then exposed to Zika, there were no signs of enhanced Zika infection seen in these macaques themselves.

They then conducted biochemical analyses to identify neuroligin - 3, confirm that the protein could stimulate tumor growth in cultured samples of several kinds of human high - grade gliomas and study which signals the protein uses within glioma cells to promote their growth.

They tested the system on cell cultures, and then in live mice with breast cancer and mice with constricted arteries in their legs.

All it took to make pluripotent stem cells, they reported, was briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking the culture conditions.

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

Lu's team then cultured the edited cells, increasing their number, and injected them back into the patient, who has metastatic non-small-cell lung cancer.

They exposed the cells to proteins associated with TB and then analyzed the cell culture for expression of certain cytokines.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

Their method relied on briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking culture conditions to produce stem cells.