Sentences with phrase «then supernatants»
To remove hepatocytes, homogenates were centrifuged at 300 rpm for 3 min, and then supernatants were centrifuged at 1500 rpm for 10 min.
http://journals.plos.org/ Not exact matches
Loaded gesicles can then be collected from the supernatant and added to target cells for efficient editing with no footprint.
The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.
Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.
After 24 hours (on day 1) the mixed viral supernatant was removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.
On the following day (considered day 0) the concentrated retroviral supernatants were thawed and mixed at a 20x OCT4, 10x SOX2, 10x KLF4, 10x cMYC ratio, supplemented with fresh MEF media up to 2 ml volume (per well) and 8 ng / ml polyprene and then exposed to the HUF1 cells at 37 °C and 5 % CO2.
On day 3 the mixed viral supernatant was again removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.
Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.