There was no significant difference between the number of pollen grains of the head and
thorax safe sites (caput: p > 0.2, Z = -1.1, thorax: p > 0.4, Z = -0.9)(Fig 2B, S2 Table).
Not exact matches
The area of each of the
safe sites (areas with residual pollen) on the animals» body as well as the area of the whole insect body (exoskeleton including projecting bristles of caput = head,
thorax and abdomen) was measured from the photos with the image processing software Fiji [44], calculating from the scale paper.
The
safe sites comprised 24.5 % of the dorsal projection of the body in B. terrestris (caput: 13.4 %,
thorax: 20.8 %, abdomen: 30.2 %) and 23.5 % in A. mellifera (caput: 9.7 %,
thorax: 41.0 %, abdomen: 21.1 %)(S1 Table).
B. terrestris: overall view with arrow - shaped
safe site on the
thorax (A),
safe site above the insertion of antennae on the head (B), dorsal
thorax (triangular)(C, D), waist (E), abdomen (F, G), and ventral abdomen (H), A. mellifera: overall view with broad
safe site on the
thorax and narrow
safe site on the abdomen (I),
safe site between the eyes (J), dorsal
thorax (K), waist (L), and abdomen (M), as well as ventral abdomen (N), dorsal
thorax and abdominal
safe site under full spectrum illumination (O) and UV - illumination (P).
In S. pratensis, 20 % of the
safe site area on the bumblebees»
thorax was contaminated by the pollen - sacs and 9 % by the stigmas.
Similar
safe sites have been observed on Xylocopa sp. (dorsal
thorax)[26], Megachile ericetorum (dorsal
thorax)[48] and Euglossa imperialis (anterior face and between
thorax and abdomen)[42](S6 Table).
Other
safe sites were similar to B. terrestris between the
thorax and abdomen, mainly the middle area of dorsal abdomen, and only few pollen grains were found on the legs and the ventral side (here mainly on the abdomen near the
thorax side)(Fig 1I — 1N, S2 Video).
In both species, the head
safe site was the smallest one (all p < 0.0001 according to Tukey post-hoc tests, except not significantly smaller than the
thorax in B. terrestris: p = 0.057, Fig 2A).