At the end of the desired treatment times, cell lysates were prepared in lysis buffer (1 % NP - 40, 0.5 mM Tris - HCl (pH 7.5), 0.14 M NaCl, 5 mM KCl, 5 mM EDTA, and 1 mM phenylmethylsulfonyl fluoride) plus serine /
threonine phosphatase inhibitor cocktail.
Not exact matches
In this study, it is shown that dephosphorylation of Tyr15 triggered activation of the pp34 - cyclin complex from fission yeast, that a human protein - tyrosine
phosphatase can catalyze this event both in vitro and in vivo, and that activation of fission yeast pp34 does not require
threonine dephosphorylation.
A widespread family of serine /
threonine protein
phosphatases shares a common regulatory switch with proteasomal proteases Bradshaw, N., et al., eLife doi: 10.7554 / eLife.26111.
More recently, we elucidated an additional mechanism of tumor suppression mediated by canonical ephrin - induced EphA2 signaling (Figure 1A), which leads to inhibition of the AKT - mTORC1 oncogenic pathway through interplay of EphA2 with a
phosphatase that dephosphorylates the AKT serine /
threonine kinase.
The balance between kinase and
phosphatase activities determines the rate of phosphorylation of the insulin receptor kinase domain and several downstream targets including the phosphatidylinositol phosphates, the serine /
threonine kinase Akt1 and the mTOR.