Sentences with phrase «through cell culture»

This is the process, tested so far only at laboratory scale, of growing pork, chicken, or beef through cell culture in vats instead of raising and slaughtering animals.

Evidence of infection and replication of the two principal viral groups that infect humans was demonstrated for multiple passages of viruses through cell culture (Figure 1).

Not exact matches

By analyzing chemical changes of the IRS - 2 protein in immortalized cultures of human white blood cells, it determined that IRS - 2 appeared in two different forms — «on,» which allows the signal to pass through, and «off,» which stops the signal from activating the cells into M2 macrophages.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

Dendritic cells are too large to pass through the blood - brain barrier, which is one reason they have to be cultured with brain tumor cells outside the body, then injected back into the patient's skin as a vaccine.

But boys who haven't been through puberty can only have sperm stem cells removed and frozen in anticipation of technology that could culture the cells and place them back in the testes, where they produce sperm after puberty.

VTT Technical Research Centre of Finland is developing a new and promising method of producing healthy and tasty plant - based food through plant cell culture (PCC) technology rather than field cultivation.

Our system runs in a normal cell culture dish, and there are no pumps; we use pressure heads to perfuse media through the vasculature.

At the time, culturing stem cells was notoriously hard, but after combing through previous lab experiments, another postdoc in Clevers's lab, Toshiro Sato, concocted a mix of growth factors that coaxed the gut stem cells to replicate in a dish.

Researchers rushed to the building the morning after the quake to wade through chemical - laced water and salvage cell cultures, enzymes, antibodies, and other frozen samples and reagents, bringing some home to their kitchen freezers.

«But one has to actually define the [culture] conditions and walk the cells through this very careful choreography.»

By culturing human stem cells in this manner and treating them with specific signaling molecules, the investigators were able to guide cells through key processes involved in the development of the human inner ear.

IPS cells are grown in culture from body cells, through the addition of genes that cause them to revert to pluripotency — the stage in which they can potentially develop into any type of body cell.

When the scientists disrupted passage through that pore in cell cultures, the parasite stopped growing and died.

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

This must be accomplished by using a process known as germ - line transmission, where the primordial germ cells (PGCs) of an early embryo are edited, grown in cultures, and then reproductively transmitted through surrogate host parents to generate live, engineered birds.

The culture fluid from infected cells was clarified by low - speed centrifugation, filtered through a 0.45 µm filter, aliquoted, and stored at − 80 °C.

We share openly our cell culture knowledge through our PhD - qualified Cell Culture expecell culture knowledge through our PhD - qualified Cell Culture eculture knowledge through our PhD - qualified Cell Culture expeCell Culture eCulture experts.

The primordial germ cells of the donor bird's embryo are extracted, grown in cell cultures, and then transmitted through the reproductive organs of surrogate host parents (known as chimeras).

We found that canonical signaling by the EphB4 receptor is low in breast cancer cells and that ephrin - induced stimulation of EphB4 kinase activity inhibits breast cancer cell malignancy in culture and tumor growth in vivo (Figure 1A) through inhibition of the CRK proto - oncogene.

Although originally studied primarily in two - dimensional in vitro assays, most cell migrations in vivo occur in complex three - dimensional tissue environments that are difficult to recapitulate in cell culture or ex vivo Further, it is now known that cells can mobilize a diverse repertoire of migration modes and subcellular structures to move through and around tissues.

The iPS cells are cultured on the opposite side of the membrane in the other channel that represents the glomerulus» urinary compartment, where they are induced to form a layer of mature podocytes that extend long cellular processes through the pores in the membrane and contact the underlying endothelial cells.

Our approaches include gene discovery, mapping of signaling networks in cell culture models and in vivo functional analyses done through gene knock - out and knock - in techniques in mice.

Results: During in vitro culture period, the percentages and absolute numbers of T cells expressing the CARs containing a hinge domain continuously increased, mainly through the promotion of CD4 + CAR T cell expansion, regardless of the single - chain variable fragment (scFv).

Subsequently, we isolated (through FACS) and cultured the top 10 % and bottom 10 % of the SSEA3 / 488 fluorescing cells as our SSEA3 - positive and negative populations respectively (Figure 2B).

Manipulating genes and then sorting through many cells to find the desired alterations wasn't possible with these cells because scientists couldn't grow them in the culture dishes where such maneuvers would take place.

Of particular importance is the BCAA leucine, a potent independent stimulator of muscle protein synthesis in cell culture and animal models through enhanced cellular regulation of mRNA translation (29).

The educational force of the wider culture is now the primary site where education takes place, what I have called public pedagogy — modes of education largely produced, mediated, and circulated through a range of educational spheres extending from the new media and old broadcast media to films, newspapers, television programs, cable TV, cell phones, the Internet, and other commercial sites.

Energy is generated through fuel cells powered with hydrogen collected from phytohydrogen generators (genetically enhanced algal cultures that produce the element as a by - product) and with solar - collector hydrogen generators.