Vince guided
them through cell lysis (soap), precipitation (salt), and then separation (ethanol).
Not exact matches
Spleens were processed into
cell suspensions
through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK
lysis buffer to remove RBCs.
Tumors can suppress CD4 + T
cell activity and CTL tumor
lysis directly
through secretion of immunosuppressive factors including TGF - β1 but also PGE - 2, and IL - 10.
Each single
cell was carried
through two rinses of PBS using a finely drawn glass capillary pipette under a dissecting microscope and then transferred in a minimal volume (0.5 µL) to a PCR tube containing
lysis buffer, protease, tRNA and poly - T gripNA ™ probe (mTRAP ™ midi - kit, Active Motif, cat.