By using random series of 30 nucleotides — the building blocks of RNA and DNA — he thought he could give neurons unique barcodes, linking the barcodes across synapses, then create maps of their connections using high -
throughput gene sequencing technology.
Not exact matches
Choudhury used high -
throughput sequencing technology to find the targets of DAZAP1 and decipher whether DAZAP1 would bind to the exons and introns of
genes.
To this purpose, GenProfile utilizes a powerful
technology platform, which involves proprietary high -
throughput technologies for
gene sequence comparison (e.g., multiplex
sequence comparison), genotyping (MALDI - TOF minisequencing), unique approaches to data analysis and interpretation, as well as a large network of clinical collaborators.
Research Focus: I dedicate my time and effort to develop high -
throughput sequencing technologies such as single cell transcriptomic, ChIP - Seq, ChIA - PET, and many more... The scientific rational is to understand better and with a different angle, the mechanisms of epigenetic regulation of
gene expression in (rare) immune cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis...
Short - read, high -
throughput sequencing technology can not identify the chromosomal position of repetitive insertion
sequences that typically flank horizontally acquired
genes such as bacterial virulence
genes and antibiotic resistance
genes.