The enrichment method employed produced an almost homogeneous population of iPS - RPE cells at passage 2 in a 25 cm2
tissue culture flask (Fig. 1C) with no evidence of cell multi-layering.
Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2
tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).
The cells were routinely cultured in 25 - cm2
tissue culture flasks containing RPMI (ATCC, 30 - 2002) supplemented with 10 % fetal bovine serum (Biochrom) according to the supplier's instruction.
Not exact matches
In 1993, Dr. Wood began working with medical scientist Marie Stoner on a method to grow skin
tissue directly on patients instead of in a
culture flask.
For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on
tissue culture treated
flasks.