The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and
tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.
Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or
tissue lysates.
Rat brain
tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.
Not exact matches
Samples matrixes suitable for metabolomics analyses include in vitro: cell
lysate and medium, and in vivo: Plasma, serum, urine, CSF, micro-dialysate and
tissues
The assay has been validated for use with human and mouse cell
lysates and
tissue homogenates, such as peripheral blood mononuclear cell (PBMC)
lysates, and will be marketed through Enzo's worldwide sales and marketing group.
Cell
lysates of hippocampal
tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).
Cell
lysates of either hippocampal
tissue or entorhinal cortex
tissue from aged AD mice on control diet (AD Ctl) or J147 diet (AD J147) were analyzed by Western blotting and the images quantified in bar graphs accompanying the images.
Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein
lysates from a limited number of human
tissues and cell lines.