Sentences with phrase «tissue microarray»

The tissue microarray method used within the human protein atlas enabled the global mapping of proteins in the human body, including the brain.

Patients and Methods: A tissue microarray (TMA) consisting of four biopsy cores from 34 matched pairs of nephrectomy and metastatic sites of clear cell RCC was used to assess PD - L1 expression by quantitative immunofluorescence.

Each neoplasm was part of a tissue microarray constructed as described elsewhere (22).

The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ hybridization FISH, laser capture microdissection, and tissue microarray construction.

On a study funded by the National Science Foundation and Agilent Technologies, researchers employed spatial light interference microscopy (SLIM), a label - free method, to perform localized measurements of light scattering in prostatectomy tissue microarrays.

Tissue microarrays are used to show antibody staining in samples from 144 individuals corresponding to 44 different normal tissue types, and samples from 216 cancer patients corresponding to 20 different types of cancer (movie about tissue microarray production and immunohistochemical staining).

Services include phlebotomy, tissue microarrays, collection of pathological specimens and support for clinical studies.

This is accomplished by combining high - throughput generation of affinity - purified antibodies with protein profiling in a multitude of tissues and cells assembled in tissue microarrays.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

And he had some preliminary evidence: When he added DNA from malignant prostate tissue cells to a microarray, a novel retrovirus emerged.

The researchers analyzed microarray data of samples from German patients and from an IPF cohort of the Lung Tissue Research Consortium in the U.S.

The Ogretmen laboratory screened previously reported microarray data sets of several human tumor tissues (metastatic head and neck squamous cell carcinoma, melanoma, and renal cell carcinoma) and showed that, in these samples, only the levels of CerS4 were significantly decreased.

To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide microarray analysis of lung tissue taken from zinc - deficient mice with sepsis.

Typical microarray - based gene expression analyses compare gene expression in adjacent normal and cancerous tissues.

Then they studied the mice's lungs microscopically and, using microarrays, measured the expression of many dozens of genes in their lung tissue at 1, 3, and 5 days after infection.

The accumulation of adipose tissue macrophages in direct proportion to adipocyte size and body mass may explain the coordinated increase in expression of genes encoding macrophage markers observed in our microarray expression data.

To identify transcriptional patterns that correlate with body mass, we used oligonucleotide microarrays to catalogue gene expression levels in the parametrial or epididymal adipose tissue from two dozen mice whose body mass and adiposity varied due to diet, sex, and mutations in genes affecting energy homeostasis.

(A) Location of the eye field (EF), PNP, and LE tissue isolated from stage 15 Xenopus embryos for microarray analysis.

Target preparation and microarray hybridization — Total RNA was extracted from retinal samples using RNeasy Lipid Tissue Mini Kit (Qiagen) and was the substrate for amplification and labeling using a procedure based on the Eberwine protocol [49].

One way is to identify transcription factors, and one way to identify transcription factors in microRNAs that can be used for reprogramming, is to screen for them through microarrays or RNA sequencing within the embryonic brain tissue.

AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

We apply FARO to a compendium of 242 diverse Arabidopsis microarray experimental factors, including phyto - hormones, stresses and pathogens, growth conditions / stages, tissue types and mutants.

A microarray study of post-mortem mRNA degradation in mouse brain tissue.

The aim of his research was to use microarray gene expression data in order to characterize ageing in different human tissues and identify the age at which major changes in genetic expression profiles occur.

Archival prostate tissue corresponding to the previously XMRV - positive VP62 sample (QQ) and derived from the same tissue block used in the original 2006 Urisman et al. study [1], further referred to as VP62 (2012), was also available for microarray screening.

Despite comprehensive microarray, PCR, FISH, and serological testing, XMRV was not detected in any of the newly collected samples or in archival tissue, although archival RNA remained XMRV - positive.