Sentences with phrase «to label the cells»

This will involve labeling cells in the growing embryo tail and using microinjections of dye to follow the pathways that these cells take as the tail develops.

Mouse heart with two sets of labeled cell clones (red and green).

Perin also used antibodies to label cells expressing vimentin and desmin, proteins found on developing, not mature, heart muscle cells.

However, such multimerization capabilities should not interfere with the intended goal of non-toxically labeling cells for deep tissue and intravital imaging as the Amrose variants 1 — 3 were developed in vertebrate cells.

The Bulte Lab has developed methods to label cells magnetically using tiny superparamagnetic iron oxide nanoparticles in order to make them visible by magnetic resonance imaging; this technology has now been introduced in the clinic for several cell therapy applications.

Historically, cell lineage tracing studies have taken large pools of cells from mature tissues and inferred their origins retrospectively by staining the cells with vital dyes or transplanting isotopically labeled cells into donor animals.

After labelling the cells so...

Winter's team learned that the cancer diagnostic market — for reagents that can simultaneously label dozens of so - called biomarkers on tumor cells — was nearly 30 times larger than her products» basic research market — for compounds to simply label cells.

«We are now able to get highly efficient brain - wide delivery with just a low - dose systemic injection, access neurons in difficult - to - reach regions, and precisely label cells with multiple fluorescent colors to study their shapes and connections.»

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

Next, the researchers labeled cells in 3 - day - old embryos and followed where they wound up in the chick.

Semiquantitative analysis revealed that approximately half (73/138) of FluoroGold - labeled cell bodies exhibited GFP label, whereas in the ganglion as a whole, including both gustatory and nongustatory innervation, a significantly lower proportion of ganglion cells exhibit GFP fluorescence [139/468; χ2 (1, n = 606) = 25.21, p < 0.0001 Pearson's χ2 test; Fig. 2D — G].

By analysing the number of fluorescently - labelled cells per clone using mathematical modelling, the team was able to show that only clones derived from mutant stem cells were able to overcome a mechanism known as «apoptosis», or programmed cell death, and continue to divide and proliferate unchecked, developing into a form of skin cancer known as basal cell carcinoma.

The green - labelled cells show a basal cell carcinoma in mouse tail epidermis derived from a single mutant stem cell and expanding out of the normal epidermis stained in red.

It was at this stage that highly purified populations of endothelial cells could be obtained by adjusting the gating parameters to select only the most intensely labeled cells.

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

Also, using magnetic metal allows us to detect only one type of labeled cell at a time.»

Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types to do more than just label cells.

Control samples included unlabeled cells, cells labeled with secondary antibody only and single fluorochrome labeled cells.

As long as two neurons join at a synapse, trans - Tango allows scientists to label the cells connected to a starter neuron, experiments in the paper show.

For example, this downloadable method compares the cell counting feature of the Spark multimode microplate reader with a GFP - labeled cell count.

By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).

Also, I was pleasantly surprised when I woke up Saturday morning to find that some of my tomato plants I planted last weekend had sprouted!!!!! I was super nervous they weren't going to work out because I made a huge mess planting them and forgot to label the cells in the planter.

IMAGE COURTESY OF DR. FINKBEINER, GLADSTONES INSTITUTES AND UCSFMicrographs of fluorescently labeled cells are undoubtedly beautiful, but they require invasive and sometimes disruptive or deadly protocols to get their glow.

In Thy1 - PE labeling experiments, Ccr6 KO mice showed reduced frequencies of labeled cells, a result that was most significant for the Vγ4 + population (Figure 4G).

Dr. Bulte has pioneered methods to label cells magnetically, making them visible by magnetic resonance imaging (MRI).

Gould's team also noted a stress effect: Monkeys housed in close quarters with other monkeys ended up with one - third fewer BrdU - labeled cells.

It turned out that 80 % of the BrdU - labeled cells were also tagged with the enolase, confirming that the newborn cells were in fact neurons.

The new AAV vectors can also deliver genes that code for colorful fluorescent proteins; such proteins are useful in identifying and labeling cells.

But Grover notes that existing techniques that fluorescently label cells can sort through millions in an hour.

The researchers solved this problem by developing markers to label the cells of interest and then testing different enzymes and other reagents to draw them out of tissues.

After they had injected the labelled cells to mice, they tracked their locations in various phases of the infarct.

They labelled the cells in the test tube with a radioactive substance whose radiation can be measured and visualized in images.

Then they labeled cells in even earlier embryos to track where they wound up.

Over the next several weeks, the shape and behavior of the fluorescent green - labeled cells were observed to see if there was evidence of regeneration.

Targeting the labeled cells for analysis, they revealed that their organoids contained a population of sensory cells that have the same functional signature as cells that detect gravity and motion in the human inner ear.

After 24 hours, labeled cells were counted under the fluorescent microscope.

The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.

Arrows indicate double - labeled cells (above threshold in each channel).

We also observed some YFP - labeled cells in the epidermis, forebrain, and occasionally olfactory bulb (Figure 4E and 4F and unpublished data).

Percent of BrdU - labeled cells were determined by dividing their number by the total number of nuclei (DAPI).