Sentences with phrase «to label the probes»

Three oligonucleotides, two primers, and an internal fluorescence - labeled probe for B. burgdorferi (strain N40) ospA and oppA - 2 target genes were designed with Primer Express software (Thermo Fisher Scientific).

In a new study from the laboratory of Gladstone Investigator Katerina Akassoglou, PhD, scientists reveal in animal models that the heightened activity of a protein called thrombin in the brain could serve as an early indicator of MS.. By developing a fluorescently labeled probe specifically designed to track thrombin, the team found that active thrombin could be detected at the earliest phases of MS — and that this active thrombin correlates with disease severity.

(c) Nuclear extracts were incubated with the biotin - labeled probes alone (middle lane) or 10-fold molar excess of non-biotin labeled wild - type probes or mutant probes.

With the aid of a magnetic plate holder, capture beads containing the hybridized mRNA were washed and incubated with provided labeling probes.

Imaging individual mRNA molecules using multiple singly labeled probes.

Using a technique called smFISH (single - molecule fluorescence in situ hybridization), the team attached dozens of small fluorescently labeled probes to each molecule of specific messenger ribonucleic acid (RNA), which causes these molecules to light up under the microscope.

Multiplex QPCR reactions of RT product were performed using FAM - labeled probes for aquaporin 4, S100b, CX43, GLT - 1 and AKAP12, in combination with VIC - labeled, primer limited GAPDH probe and Taqman mastermix (all Applied Biosystems).

You don't have to be (or collaborate with) an RNA in vitro transcription expert to make your labeled probes.

Dual - Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.