FoxP3 expressing cells that were CD4 + were readily detected in
tolerant allografts (Figures 7c — e).
As expected, acutely rejected allografts were massively infiltrated by mononuclear cells, whereas
tolerant allografts were free of infiltrating cells at both 40 and 100 days post-transplantation.
In
the tolerant allograft, FoxP3 + cells (C) and CD4 + (D) cells were detected, which overlapped when merged (E) suggesting that the CD4 + cells expressed FoxP3.
Not exact matches
MSC - induced Tregs were donor - specific since adoptive transfer of splenocytes from
tolerant mice prevented the rejection of fully MHC - mismatched donor - specific secondary
allografts but not of third - party grafts.
By day 100, however, mixed chimerism was undetectable in peripheral blood despite the fact that the recipient animals were
tolerant to the
allografts.
CD4 + T cells sorted by immunomagnetic beads from splenocytes of animals
tolerant to donor - type cardiac
allografts were plated at 106 cells / well.