Sentences with phrase «tonsillar lymphocytes»

Human tonsillar lymphocytes were treated with lethal toxin or E687C mutant lethal toxin (1.0 μg / ml) for 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hr.

For initial lethal toxin experiments splenocytes / tonsillar lymphocytes were cultured in media containing in 0.1 % serum to minimize the effect of serum on lethal factor enzyme activity.

Human tonsillar lymphocytes were treated with increasing concentrations (0.01 — 10 μg / ml) lethal toxin for 3 hrs followed by IL - 23 (50 ng / ml) stimulation for 18 hr.

(A) Human tonsillar lymphocytes or (B) sorted Lin - CD127 + cells were pretreated with lethal toxin (1 μg / ml) for 2 — 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hrs.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

CD19 depleted - tonsillar lymphocytes were sorted for CD3 - CD19 - CD14 - CD56 - CD127 + ILCs.