Sentences with phrase «total rna»

The total RNA from the adipocytes was isolated and carried out GeneChip microarray analysis.

Total RNA from 8 to 18 embryos was extracted using Omega E.Z.N.A Total RNA Kit (Norcross, Georgia).

2 μg of the total RNA was reversely transcribed using a High Capacity RNA - to - cDNA Kit (Applied Biosystems, Foster City, CA).

Three micrograms of total RNA was used for the polyA + library construction using a TruSeq SBS Sequencing Kit, version 3.

Total RNA was extracted from cultured cells with RNeasy Mini kit (Qiagen), with the use of QIAshredder spin column for homogenization and an on - column DNase digestion.

No. 18080051, random hexamer priming) was used to generate cDNA starting from 1.5 μg of total RNA.

Subsequently, equal amounts of total RNA (300 - 500 ng) were reverse transcribed into cDNA using random primers (SuperScript First Strand Synthesis for RT - PCR; Invitrogen, Carlsbad, CA).

RNA was prepared from testis of control and IVC - produced 9 - mo - old mice, using the ULTRASPEC total RNA Isolation Reagent Kit (BioTex Laboratories, Inc.) according to the manufacturer's instructions (two animals pooled per sample, with triplicated samples).

Frozen total RNA from any human sample and / or human derived cell - cultures.

Total RNA was isolated using the RNeasy Mini Kit from Qiagen and treated with RNase - free DNase I (5 units / 100 µg of nucleic acids, Sigma).

Total RNA extracted from prostate cancer tissue cores was tested in the single - round XMRV pol RT - PCR assay utilizing the m2000rt system (Abbott Molecular, Inc.; Desplaines, IL).

Total RNA was isolated from iPS, iPS - RPE and foetal RPE cell cultures using the Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA).

An RT - PCR assay for the detection of mouse IAP sequences was also performed on total RNA extracts from VP35 and VP42 and polyA RNA extracts from VP62 (2006)(as no total RNA was available) using previously published primers and conditions [42].

The quality and quantity of total RNA were monitored and measured with a NanoDrop (NanoDrop Technologies) following the manufacturer's instructions.

For the analysis of gene expression in blastocysts, total RNA was isolated and purified from three pools of 10 blastocysts produced in vivo or in vitro (KSOM supplemented with 10 % FCS or in the presence of 1 g / L BSA) using the RNeasy Micro Kit (Qiagen)[18].

Total RNA was isolated in TRIzol from homogenized tissue and cells and purified using RNeasy columns and RNase - Free DNase digestion according to the manufacturer's instructions (QIAGEN).

An average of ∼ 500 ng of total RNA input per reaction was diluted in water to a total volume of 25 µl, and 25 µl of master mix that contained EZ buffer, rTth enzyme, dNTPs, Rox reference dye, MnCl2 and primer / probe was added to obtain a final reaction volume of 50 µl.

Samples were redissolved in 20 μl of RNase free deionized water, and the concentration of total RNA was determined by UV spectrophotometry.

Total RNA was quantified and assessed for purity using a Nanodrop spectrophotometer (Thermo Scientific).

Total RNA was isolated from 106 IL - 3 dependent BaF3 phe116, BaF3 phe116 / 9 or autonomous BaF3 (BaF3 Aut) clones using TriPure reagent (Roche) according to the manufacturer's instructions.

This method conducts the reverse transcription reaction and PCR in a single tube format from 20ng total RNA template.

Total RNA from monkey tissues was extracted using Sepasol RNAI (Nacalai Tesque, Kyoto, Japan) according to the manufacturer's instructions.

After an overnight incubation at 4 ° C, for thorough stabilization, samples were homogenized in lysis buffer and total RNA was isolated using the GE Illustra RNAspin Isolation Kit (GE Healthcare) according to the manufacturer's protocol.

General speaking, microarray requires high - quality intact total RNA as starting material for target preparation in mRNA profiling.

For microRNA expression profiling, however, we need whole total RNA without any column purification because mature microRNA in sizes 19 ~ 22nt and their precursors in sizes 60 ~ 110nt can be lost after column purification.

Reverse transcription was performed on 1 μg of total RNA with an oligo (dT) primer (Roche) and M - MLV RT (Invitrogen).

Total RNA from ES cells or EBs was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany).

Total RNA from mutant and wild - type ovaries was isolated using Trizol (Invitrogen).

Total RNA was reverse transcribed with RT2 First Strand Kit (SABiosciences) according to the manufacturer's instructions.

For this analysis, total RNA extracted from whole larvae was used, as dilp2 and dilp5 are predominantly expressed in the CNS during the larval stages examined [26,27].

cDNAs were prepared by reverse - transcribing 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo), and quantitative RT - PCR was performed using Thunderbird qPCR Mix (Toyobo).

The extracted total RNA was stored at − 80 °C until use.

For cell lines, early - split samples were used as duplicates and total RNA was extracted using the RNeasy mini kit.

Total RNA from whole larvae or larval tissues was extracted using a PureLink RNA Mini Kit (Life Technologies).

Cells were harvested, and RNA was isolated using the RNeasy kit (Qiagen); 1 μg of total RNA input was used for each sample.

After DNase treatment, total RNA concentration was quantified and 1 μg total RNA was converted to cDNA using oligo dT and reverse transcriptase.

The extracted total RNA was subjected to two separate reactions — one to synthesize cDNA and the other to test for DNA contamination.

Total RNA was extracted using TRIzol (Invitrogen) from either the fat body or whole body.

Total RNA was purified with RNeasy mini kits according to the manufacturer's instructions (Qiagen, Valencia, CA) using ~ 10 mg of tissue.

Total RNA was quantified using the ND - 1000 Nanodrop and assessed for quality using the ratios: A260 / 280 (range: 1.9 to 2.1) and A260 / 230 (range: 2.0 to 2.2, if < 2.0, contamination), in addition to the Bioanalyzer (Agilent Technologies, Cedar Creek, TX, USA) if further quality assessment was required.

One point two micrograms of total RNA from each cell line was reverse - transcribed using random primers and the High - Capacity cDNA Synthesis Kit (Applied Biosystems, Foster City, CA, USA).

Ten nanograms of total RNA from each cell line was converted to cDNA using the High - Capacity cDNA synthesis kit (Applied Biosystems).

Using 2.5 µg of total RNA, RT - PCR was performed with RT - Ace (Toyobo, Tokyo, Japan) according to the manufacturer's protocol.

Total RNA was isolated from each cell line using an RNA Extraction Kit (Qiagen Inc., Valencia, CA, USA) in accordance with the manufacturer's instructions.

Total RNA was isolated and cDNA was prepared as per the protocol of Venkatesan et al [16].

Total RNA was extracted from the cerebellar cultures at 14 and 16 DIV and renal fibroblast cultures using the RNeasy Mini Kit (Qiagen, Tokyo, Japan).

Total RNA extracted from hand - dissected S. viridis inflorescence primordia at 15 DAS was used to synthesize cDNA for amplification of the bsl1 - 2 transcript products, which were purified, cloned, and sequenced (Figure 3C).

Total RNA from Cellartis enhanced hiPS - HEP cells from C12, C18, and C22 (n = 2 batches per cell line) was extracted on Day 13 post-thawing as well as from hphep cells (n = 3 donors) Day 1 post-thawing using the GenElute RNA / DNA / Protein Plus Purification Kit (Sigma Aldrich).

Total RNA was isolated using the Quick - RNA MiniPrep kit (Zymo Research) with in - column DNase I treatment following the manufacturer's instructions.

Total RNA was isolated from the S. pistillata fragments by using TRIzol ® reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer's instructions.