Among
the total gene transcripts that were differentially expressed during night - time four biological processes were found: protein folding, mitochondrial membrane, innate immune system, and protein targeting (Fig. 4B).
Among
the total gene transcripts that were differentially expressed during daytime six biological processes were found: Wnt signaling pathway, cell junction organization, SMAD binding, regulation of cell morphogenesis, TGF - β signaling pathway, and amino acid synthesis (Fig. 4A).
Single - cell RNA sequencing allows researchers to determine the precise nature of
the total gene transcripts, or all of the genes that are actively expressed in a particular cell.
Not exact matches
Strikingly,
transcripts of β - casein and α - lactalbumin
genes make up 45 % of the
total pool of mRNA during mature lactation.
In order to focus on
genes that share similar biological processes and correlate with the calcification process, the analysis continued for unique up - regulated
gene transcripts and
total up - regulated
gene transcripts that were differentially expressed during daytime (437
genes for unique and 1,333 for
total), and during night - time (267
genes for unique and 1,171 for
total).
Release 1.0 contains 15,419 high confidence protein - coding
genes; alternatively spliced
transcripts derived from 992
genes add an additional 1,370 proteins yielding a
total of 16,789 predicted proteins.
Unsupervised hierarchical clustering of the 5,959
genes, using 100 random permutations, gave rise to 115 groups, containing a
total number of 2,855
transcripts, with a probability of 95 % or higher that clustering was not random (Supplemental File S2).