Sentences with phrase «total reaction volume»
The digestion was performed using BtsI CutSmart (New England BioLabs) with 2.5 µL 10X buffer, 0.40 µL BtsI, 1 µg DNA, and water adjusted accordingly for a total reaction volume of 25 µL.
http://journals.plos.org/ Not exact matches
An average of ∼ 500 ng of total RNA input per reaction was diluted in water to a total volume of 25 µl, and 25 µl of master mix that contained EZ buffer, rTth enzyme, dNTPs, Rox reference dye, MnCl2 and primer / probe was added to obtain a final reaction volume of 50 µl.
Reactions were performed in 25 µl total volume with 50 ng genomic DNA, 1.5 mM MgCl2, 10 pmoles of each primer, 0.33 mM dNTPs, and 1.5 U of native Taq (Promega, Madison, WI).