Sentences with phrase «total supernatant»

For the total input control, 20 % of the total supernatant was saved and frozen at − 80 °C.

Not exact matches

The results revealed that the supernatant of 72 - hour ATRA - treated NB4 cells was sufficient to induce the tyrosine phosphorylation of STAT2 and the endogenous RIG - G level in U3A cells, in comparison with the relative consistent level of total STAT2 (Fig. 3B).

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.