Heterotrimeric guanine nucleotide — binding protein (G protein)-- coupled receptors constitute the largest family of eukaryotic signal
transduction proteins that communicate across the membrane.
Not exact matches
These findings suggest that the therapeutic
protein was able to interact normally with its binding partners among the USH1 molecular complex (the
proteins cadherin 23, protocadherin 15, myosin VIIA and harmonin), as required for the mechanoelectrical
transduction apparatus of the hair bundle to function correctly.
Among these was the
protein VGLUT1, which is essential for signal
transduction in specific nerve cells.
The researchers examined the crystal structure of the complex of SeV C
protein and transcription factor STAT1, and found that SeV C
protein inhibits the signal
transduction pathway of interferon gamma.
It is expected that the molecular mechanisms underlying the inhibition of interferon signal
transduction by C
proteins would then be fully identified.
Outer membrane
proteins have crucial physiological and structural functions, among them nutrient acquisition, secretion, signal
transduction, outer membrane biogenesis, and motility.
Recent studies such as ours, however, establish
protein phosphatases as positive and essential regulators of signal
transduction, with remarkable substrate specificity and coordinated activities.
It is a rare autoimmune disease that results in the body producing antibodies against the NMDA receptor, a
protein that plays an important role in signal
transduction in the brain.
During the early years of my PhD studies, I was very fascinated by the exciting discoveries in the field of signal
transduction, in particular how receptor tyrosine kinases are activated to transmit their signals and how
protein complexes are formed through defined
protein folds (domains) interacting with specific cellular targets.
Signal
transduction through the T - lymphocyte CD4 receptor involves the activation of the internal membrane tyrosine -
protein kinase p56lck.
Nicholas Tonks Posttranslational modification; phosphorylation; phosphatases; signal
transduction;
protein structure and function
A well - known class of
proteins may fill in the dotted lines that connect signal
transduction pathways to the genes they regulate.
His graduate and postdoctoral work led to seven first author papers in the Journal of Biological Chemistry, including four back - to - back papers on fundamental characterization of arachidonic acid pathways, and a Cell paper, which was the first to demonstrate cAMP - independent G
protein participation in receptor - mediated signal
transduction.
Linda Van Aelst Signal
transduction; Ras and Rho
proteins; tumorigenesis; neural development and disorders
Immunophilins are now known to function at the crossroads of
protein folding and trafficking, and signal
transduction.
His academic research focused on gene regulation and signal
transduction mediated by ion channels and G
proteins.
Moffat and colleagues were the first to conduct time - resolved crystallography and developed photoactive yellow
protein as a paradigm for light - dependent signal
transduction in biology.
The scientists exploited this molecular mechanism to construct a tumour - specific probe called POL - N (
Protein transduction domain [PTD]- ODD - Luciferase - Near - infrared [NIR] dye) which contained: 1) the ODD core, 2) a PTD for intracellular delivery, 3) red - shifted luciferase, and 4) a NIR fluorescent dye with peak emission around 700 nm.
Recently, interest has developed regarding the possibility of targeting intracellular inhibitory
proteins to improve T cell activity against cancer, including diacylglycerol kinase ζ (DGKζ)(9, 10) and Casitas b - lineage proto - oncogene b (Cbl - b)(11), which attenuate signal
transduction events downstream of the TCR and CD28.
My research investigates the role of a particular
protein we initially thought was just a «dead» enzyme as a regulator in signal
transduction cascades (cellular communication).
Functional correction of T cells derived from patients with the Wiskott - Aldrich syndrome (WAS) by
transduction with an oncoretroviral vector encoding the WAS
protein.
Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A.
Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope
Proteins.
Our synthetic signal
transduction system was built using a modular assembly of bacterial and plant
protein domains.
Even though different cell types were used as the initial starting materials, and they were made to produce different sets of
proteins, both groups were able to identify and isolate hESC - like cell colonies only 20 to 30 days after
transduction.
For example, it modulates enzymatic activity, induces structural changes in
proteins and nucleic acids, alters kinetics of molecular bonds, regulates motion of molecular motors, and has a role in mechanical
transduction and sensory functions.
Homer 1, an actin binding
protein that is induced by BDNF [72], belongs to a family of scaffolding
proteins that localize at the postsynaptic density (PSD)[73, 74] and is believed to play a critical role in signal
transduction, synaptogenesis and receptor trafficking at synapses [75].
Drugs like Gleevec ®, known as signal
transduction inhibitors, work by «turning off» specific
proteins in cancer cells that cause cancer cells to grow and multiply.
CD45 is a pan-leukocyte
protein with tyrosine phosphatase activity involved in the regulation of signal
transduction in hematopoiesis.
Helicobacter pylori effector
protein CagA activates the signal
transduction and activator of transcription 3 (STAT3) signaling pathway: a potential mechanism for gastric cancer development.
In 2005, the identification of an activating mutation in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal
transduction and activation of STAT transcription factors.6 All JAKs share a similar
protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.10
Death - associated
protein kinase (DAPK) and signal
transduction: fine - tuning of autophagy in Caenorhabditis elegans homeostasis.
This work revealed new roles for XBP - 1 in regulating signal
transduction through the B cell receptor; in regulating the expression of important transcription factors in B cells; in maintaining proper lipid synthesis and
protein glycosylation in B cells; and in colonization of stimulated B cells into the bone marrow for sustention of antibody production.
His laboratory focuses on the roles of reversible
protein modifications that are involved in modulating signal
transduction, chromatin dynamics and gene activation.
It remains to be elucidated whether differences in translation and / or
protein modification of receptors or signal
transduction machinery underlie sexually dimorphic detection of pheromones.
Protein - DNA complexes were then immunoprecipitated overnight with polyclonal Oct4 antibody (sc - 9081, Santa Cruz Biotechnology), a mix of monoclonal Oct4 antibodies (BD
Transduction Labs, Santa Cruz Biotechnology, Chemicon), or normal serum controls (Pierce) on a rotator at 4 °C.
In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly used CRISPR / Cas9 delivery formats, including lentivirus
transduction, plasmid lipofection, and ribo nuclear
protein electroporation, on the dynamics of indel profile formation.
Several projects in the area of signal
transduction, fluorescent
proteins engineering, optogenetics and advanced microscopy are available.
Berberine improves insulin signal
transduction by stimulating glucose intake via the activation of the AMP - activated
protein kinase pathway.
While it is true that amino acids were long considered simply substrates for
protein synthesis, they have more recently shown to also act as modulators of intracellular signal
transduction pathways that are typically associated with growth - promoting hormones such as insulin and IGF - 1.
Additional upregulated signal
transduction related genes encoded
proteins polycystin - 1L1, MLP and LIM regulatory
proteins, fibrillins or related
proteins containing Ca2 + - binding EGF - like domains, Ca2 + / Mg2 + - permeable cation channel, and other differentially expressed putative transcripts that may play a role in cellular signaling, including known extracellular
proteins c - type lectin and collagen (see electronic supplementary material, table S5).
Tags for this Online Resume: Tissue Culture,
Protein, Molecular, Cell, Pharmacology,
Transduction, Assays, Antibody, Patent, Scientific writing, Oral communication,
Protein purification, Group leader, Immunology, Inflamation
Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines,
Protein Kinase Assay, Pulse Chase Experiments, Transfection,
Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology
Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS