Sentences with phrase «transduction with»

Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a subpopulation of cells that experessed human IgG, as shown by the increased fluorescence intensity.

Studies of satellite cell activation via nitric oxide have opened an exciting area of research on muscle regeneration, growth and the muscle - fiber cytoskeleton that integrates mechanical and biochemical signal transduction with cell and molecular biology of muscle tissue in growth, development, disease, aging and evolution.

Functional correction of T cells derived from patients with the Wiskott - Aldrich syndrome (WAS) by transduction with an oncoretroviral vector encoding the WAS protein.

Not exact matches

These findings suggest that the therapeutic protein was able to interact normally with its binding partners among the USH1 molecular complex (the proteins cadherin 23, protocadherin 15, myosin VIIA and harmonin), as required for the mechanoelectrical transduction apparatus of the hair bundle to function correctly.

«Dr. Smith, I'm soon going to be leaving the biochemistry program here at Purdue with a Ph.D. involving signal transduction, and I am wondering if you might make a recommendation of some contacts at your company whom I could talk with.

«Science Signaling: Research, Teach, and Discover with the Weekly Journal Devoted to Cellular Signaling Transduction» [PDF, 3.5 MB] Nancy Gough, Ph.D., Editor, Science Signaling, AAAS / Science

In addition to the increased collaboration, the intellectual critical mass existing within the IMCB also helped the learning process through stimulating discussions with seasoned experts in exciting topics such as signal transduction and cell cycle processes.

FosB is a transcription factor in the brain which, together with other molecules, is involved in so - called signal transduction (transmission of stimuli to the cells), that is to say conveys genetic information between the cells and also determines whether certain genes are activated or not.

Recent studies such as ours, however, establish protein phosphatases as positive and essential regulators of signal transduction, with remarkable substrate specificity and coordinated activities.

During the early years of my PhD studies, I was very fascinated by the exciting discoveries in the field of signal transduction, in particular how receptor tyrosine kinases are activated to transmit their signals and how protein complexes are formed through defined protein folds (domains) interacting with specific cellular targets.

Among the most interesting ones identified were those associated with 1) the large category of transport, and the more specific categories of carbohydrate transport and cation: amino acid symport; 2) several related to calcium, including calcium ion binding, calmodulin binding, and voltage gated calcium channel activity; 3) DNA damage response and signal transduction; 4) response to oxidative stress; and 5) oxygen and reactive oxygen species metabolism.

These cells were immortalized by retroviral transduction of the SV40 T antigen and Telomerase reverse transcriptase (TERT), the catalytic subunit of the telomerase complex [4], and then termed HMLE (Human Mammary with Large T and TERT).

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

Transduction of the mammary epithelium with adenovirus vectors in vivo.

The scientists exploited this molecular mechanism to construct a tumour - specific probe called POL - N (Protein transduction domain [PTD]- ODD - Luciferase - Near - infrared [NIR] dye) which contained: 1) the ODD core, 2) a PTD for intracellular delivery, 3) red - shifted luciferase, and 4) a NIR fluorescent dye with peak emission around 700 nm.

She did her PhD on the transduction of the Notch signal in mammals in Alain Israël laboratory at the Pasteur Institute, Paris; she realised her postdoc with Iva Greenwald, at Columbia University NYC, on vulva organogenesis in C. elegans.

The current project will use high - resolution mass spectrometry - based metabolomics approaches to measure temporal and spatial changes of metabolic pathways in the RPE and retina under both physiological and disease conditions, with a focus on mTOR - mediated signal transduction pathways.

Molecular mechanisms of heart failure and arrhythmogenesis with focus on signal transduction

(A) GUS activity with and without 10 µm of the TNT ligand in paired leaves from transgenic plants serving as negative controls and transgenic plants containing the complete signal transduction system.

In these plants, there is no significant difference in the GUS activity with or without the TNT ligand, indicating that the complete signal transduction system and phospho - relay through PhoB - VP64 is required for transcriptional activation.

Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.

In addition, because we developed PhoB - VP64 for plants by activating endogenous HKs with exogenous cytokinins [12], it is likely that our synthetic signal transduction system (receptor → HK → PhoB: VP64 → signal receptive promoter) has false input or cross-talk from plant HK components.

Approaches successful in other systems, such as modeling signal transduction and synthetic biology methodologies [32] could improve future generations of detector plants by enhancing signal transmission and providing means to deal with biological «noise».

With refinement of signal transduction components, stronger and more specific signaling pathways may be possible.

CD45 is a pan-leukocyte protein with tyrosine phosphatase activity involved in the regulation of signal transduction in hematopoiesis.

We study the signal transduction pathways regulating cell growth and proliferation, with an emphasis on kinase enzymes.

In this study, we established mouse iPS cell lines from NPCs of neonatal EGFP - transgenic mice on the HFF feeder cells via viral transduction of 4 factors, combined with application of 2i.

Joining Dr Adeli today is Sambhav Dave, the Senior Associate in the Business Development team, with a primary focus in Neuroscience and Signal Transduction research areas.

Vorinostat interferes with the signaling transduction pathway of T - cell receptor and synergizes with phosphoinositide - 3 kinase inhibitors in cutaneous T - cell lymphoma

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

In 2005, the identification of an activating mutation in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.10

We discovered several Eph receptors and ephrins, and research in our laboratory is dedicated to the characterization of Eph receptor signal transduction mechanisms and biological functions using biochemical, mass spectrometry, molecular biology and cell biology approaches in conjunction with animal models.

Introducing new DNA often is achieved by viral transduction of target cells with a therapeutic transgene, through either a direct or cell - based gene therapy modality.

Structures of the first representatives of Pfam family PF06938 (DUF1285) reveal a new fold with repeated structural motifs and possible involvement in signal transduction.

For adenoviral transduction, HB1.F3 cells were incubated with purified adenovirus (1 ∶ 1000 dilution in DMEM) for 4 h and then cultured overnight in DMEM.

Protein - DNA complexes were then immunoprecipitated overnight with polyclonal Oct4 antibody (sc - 9081, Santa Cruz Biotechnology), a mix of monoclonal Oct4 antibodies (BD Transduction Labs, Santa Cruz Biotechnology, Chemicon), or normal serum controls (Pierce) on a rotator at 4 °C.

The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot viral transductions, and overviews for beginners just starting work with virus.

His laboratory is currently focused on characterizing signal transduction pathway abnormalities in prostate cancer, with an eye toward translational implications.

BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

Our finding that Gasp constitutively associated with Grb2 is quite intriguing and provides some possible links to hypothesize its function in positive selection, because Grb2 is an important adaptor for Sos in the TCR - mediated signal transduction pathway.

Once inside the cell, it can interfere with bacterial cell signal transduction and gene transcription processes.

However, there is evidence from recent studies that one antimicrobial effect in bacteria is related to monolaurin's interference with signal transduction (Projan et al 1994), and another antimicrobial effect in viruses is due to lauric acid's interference with virus assembly and viral maturation (Hornung et al 1994).

While it is true that amino acids were long considered simply substrates for protein synthesis, they have more recently shown to also act as modulators of intracellular signal transduction pathways that are typically associated with growth - promoting hormones such as insulin and IGF - 1.

Plasma adiponectin is positively associated with enhanced insulin signal transduction in skeletal muscle [38].

Finally, it must be acknowledged that our data are potentially limited by providing only a single «snapshot» during recovery and the possibility exists that our muscle biopsy time - points failed to coincide with peak phosphorylation responses of signal transduction.

Nick was open and honest with... me while the transduction was going on he even had some other futures installed on my truck.

Programs with research emphasis on signal transduction in development and oncogenesis, cell adhesion molecules in development and oncogenesis, muscologskeletal atrophy and countermeasures, ovarian follicle development, neural regeneration, implantable therapeutic or diagnostic devices and application of computers in veterinary education.