Sentences with phrase «transient gene expression»

We combine biochemical, structural, cellular and functional information using purified proteins, mutant and transgenic plants, yeast and chemical genomic screening systems, transient gene expression assays, confocal microscopy and in silico data analysis to compare ROP - centered kinase signaling during cell polarity (in vitro pollen tubes), morphogenesis (whole plant) and pathogenesis (fungi - infected cells).

«Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development» (DOI: 10.3389 / fpls.2015.01169)

Another provocative observation from the new study was that transient gene expression events during brain development set up broad distinctions in neural fate between cells in different areas in the cerebral cortex.

Not exact matches

A significant advantage of ZFN gene modification, compared to retrovirus based approaches, is that only transient transgene expression is required to permanently engineer an HIV resistant cell.

In particular they observed for the first time a transient pulse of gene expression that precedes the segmentation of the human embryo into discrete parts that will become the head, trunk and limbs of the body.

We notably follow the time course of structural changes in response to cues that affect gene expression either transiently or permanently: changes in genome structure during transient hormonal response of differentiated cells and stable trans - differentiation of B cells to macrophages.

By looking at the gene expression profile in single cells, the researchers were able to identify previously unknown transient states that typified the progression from precursor to more - specialized cells.

Adenoviruses, which are part of a separate family, can also infect non-dividing cells and are generally used to achieve transient high gene expression.

If such seemingly transient changes persist, they may alter the expression of genes needed to ward off cancer and other diseases.

For example, the transient expression of cluster 30 genes at day 3 in vitro, which corresponds to embryonic day E6.5 (3.5 +3), matches the expression timing of genes such as Cerberus - like 1 and Wnt3 in vivo [48], [49].

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS