Sentences with phrase «treat cultured cells»

To test this theory, they identified a short peptide that inhibits the binding of cyclin A-cdk2 to E2F and used it to treat cultured cells.

Not exact matches

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

They found high levels of the regulatory T - cells in patients treated with post-transplant cyclophosphamide, and lab - cultured cells survived cyclophosphamide treatment.

Finally, in cell cultures and animals, overexpressing the hormone could produce something that looks like cancer, and that would shrink when treated with an IGF - 1R blocker.

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been using cultured mesenchymal stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.

«We've taken white cells from his body, put them in a culture with malignant cells, and they gobble them up,» says Michael O'Rourke, who is treating the patient.

In this study, Nicchitta and his colleagues treated tissue culture cells with a stress - inducing agent called thapsigargin.

In animal and cell culture studies, the drug inhibited growth both in estrogen - dependent breast cancer cells and in cells that had developed resistance to the anti-estrogen tamoxifen and / or to the aromatase inhibitors, two of the most widely used types of drugs to prevent and treat estrogen - dependent breast cancer.

Next, Grima looked at cell death in cultured neurons with a healthy or a mutant form of Huntingtin, or with a mutant form of Huntingtin that was treated with small amounts of an experimental drug called KPT - 350, one that prevents a nuclear export protein, Exportin - 1, from shuttling proteins and RNA out of the nucleus.

An important approach in advanced cancer medicine is to treat cancer using a patient's immune cells after first arming the cells against the tumor in culture.

The researchers verified their findings by treating cultured pancreatic cancer cells with recombinant MMP3.

A new study shows coibamide A has potent anti-cancer activity in mice and cell cultures that model brain tumors and triple negative breast cancer, two of the most aggressive and difficult - to - treat types of cancer.

By culturing human stem cells in this manner and treating them with specific signaling molecules, the investigators were able to guide cells through key processes involved in the development of the human inner ear.

Normally, CD4 cells will start dividing when they are treated with certain proteins in a culture dish.

At the time, his varied interests — in the use of skin cell culture to treat burns, in human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.

Furthermore, in control cultures that were untransduced or treated with R5 - ZFNs, viral titers exponentially increased until extensive cell death began approximately 8 — 10 days post infection.

An oral drug used to treat an illness unrelated to HIV eradicated infectious HIV - producing cells in lab cultures while sparing uninfected cells — and suppressed the virus in patients during treatment and for at least eight weeks after the drug was stopped, according to results of a clinical pilot trial and researchers at Rutgers University and Dartmouth College.

We also found that after 19 days post-HIV infection, reduced but significant cell growth was detectable in several of the HIV - infected control cultures, untransduced and treated with R5 - ZFNs.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

However, this is unlikely given that the frequency of in - frame mutations remained stable over nearly four weeks in culture, that there was no significant increase in cell death between control cultures and those treated with X4 - ZFNs, and that the most common in - frame mutant was not expressed on the cell surface and thus can not maintain functionality.

In contrast, in cultures treated with X4 - ZFNs viral titers steadily decreased after peak viremia while cell growth remained exponential suggesting there was not significant viral production (data not shown).

To determine the causes of this depletion, the investigators treated neurons grown in cell culture with amyloid - beta proteins, which accumulate in Alzheimer brains.

None of these toxic effects were observed when the same cells were treated with culture medium from non-senescent astrocytes that had not been subjected to paraquat.7

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

Confluent HFF cells were treated with irradiation of 55 Gy and plated onto culture dishes (1 × 105 per 2.89 cm2).

ROS levels, as measured by the proportion of cells that stained positive for CellROX, increased from an average of 8.1 % in cultures treated with DMSO to 78 % in cultures treated with curcumin.

Where indicated, cells were treated daily with 10 or 200 ng / ml of growth differentiation factor (GDF) 5 (GDF5)(Biopharm GmbH), which are maximal saturating concentrations in primary and SH - SY5Y cell cultures respectively [17,41].

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

HES3 cultures were treated with 10 µM Y - 27632 (ROCK inhibitor, Axxora LLC, San Diego, CA) for 1 hour prior to dissociation to single cells using TrypLE ™ (Invitrogen, cat.

To eliminate the possibility of any undifferentiated cells in the cultures accounting for this observation, DMSO - differentiated cells were treated with the anti-proliferative agent cytosine arabinosidase (Ara - C), starting two days after aggregate plating.

Therefore, twelve day old, DMSO - differentiated, Ara - C treated P19 cultures were incubated with the proteasome inhibitors MG - 132 and lactacystin for four hours; this was sufficient to rescue Oct4 protein expression (Figure 1D), suggesting that these cells do indeed synthesize new Oct4 protein.

HEK293T cells were seeded at 2 × 107 cells in 15 - cm tissue culture plates and cells were treated with 20 mM LiCl for 7 hr.