Sentences with phrase «treated tissue culture cells»

In this study, Nicchitta and his colleagues treated tissue culture cells with a stress - inducing agent called thapsigargin.

Not exact matches

At the time, his varied interests — in the use of skin cell culture to treat burns, in human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

HEK293T cells were seeded at 2 × 107 cells in 15 - cm tissue culture plates and cells were treated with 20 mM LiCl for 7 hr.