Central human corneal endothelium («central tissue») was collected from the button cut by a 8 mm (diameter) circular
trephine.
This discordance may be related to the sample variation between
the trephine biopsy and aspirates because of heterogeneous or patchy marrow involvement, a failure to aspirate sufficient lymphoma cells, or insufficient primer homology for amplification.
In order to be certain that no activity occurred centrally, we divided the HCEC tissues of a 30 - year - old donor into central and intermediate - peripheral regions with a 4 mm
trephine.
His surgeon
trephined him 27 times — and he lived.
In the case of telomerase activity, the tissues were divided into central, intermediate and peripheral areas by the use of
trephines.
Figure 2 shows sets of results for four areas of human corneal endothelial tissue; central endothelium (defined by endothelial cells within a 4 mm
trephined boundary), central - intermediate endothelium (defined by endothelial cells within an 8 mm
trephined boundary), intermediate - peripheral endothelium (defined by endothelial cells between the 4 mm trephine edge and 12 mm from the center of the corneal endothelium), and the peripheral endothelium (defined by the 8 mm
trephined edge and 12 mm from the center of the corneal endothelium).