Sentences with word «tubulin»

Microtubules, hollow fibers of tubulin protein only a few nanometers in diameter, form the cytoskeletons of living cells and play a crucial role in cell division (mitosis) through their ability to undergo rapid growth and shrinkage, a property called «dynamic instability.»

IHC image of ab7291 staining alpha Tubulin in human colon formalin fixed paraffin embedded tissue sections *, performed on a Leica Bond.

ICC / IF image of beta Tubulin staining in HeLa cells.

Like the family of taxanes (of which paclitaxel is a member), epothilones prevent cancer cells from dividing by interfering with tubulin proteins that form the cells» skeletal microtubules.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Here, FGFR - TACC (shown in red) can be seen interacting with tubulin bundles (green), structures that support mitosis, at the point connecting the two daughter cells (whose nuclei are colored blue).

Immunohistochemistry of paraffin - embedded human breast cancer tissue slide using 66240 -1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens)

It blocks (by 70 - 80 %) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

These findings add to the growing understanding of the «tubulin code» — a phenomenon where TTLL7 and similar proteins add amino acids to microtubules and prompt them to fast - track certain proteins for transport.

Then they put all six genes into a single piece of DNA, so that they would all be transcribed together — and found that they could reconstitute the «3 - D printer» that assemble tubulin dimers.

Now, a new study from University of Georgia cellular biologists shows the mechanism behind tubulin transport and its assembly into cilia, including the first video imagery of the process.

GTPase activity — coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis.

To achieve this, the scientists fused SiR molecules with compounds that bind tubulin or actin.

In the center, FGFR - TACC fusion protein (red) can be seen disrupting tubulin bundles (green), structures that support cell division, or mitosis, at the point connecting the two daughter cells (whose nuclei are colored blue).

The labelling is for acetylated tubulin in red (identifying all axons), and green for the cell permeable dye calcein, which is only applied on the axonal side of the chambers (top half) and allows the identification of those neuronal cell bodies (bottom half) that have extended axons to the other side of the microfluidic device.

Julie Craft is a sixth - year doctoral student at the University of Georgia and is studying the mechanism behind tubulin transport and its assembly into cilia.

Immunohistochemistry (Formalin / PFA - fixed paraffin - embedded sections)- Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)

For example, formation of the mitotic spindle significantly depletes the numbers of free tubulin molecules in the soluble phase of the cytoplasm.

The energy maybe required to build stable alpha and beta tubulin assembly.

Things like HIV protease inhibitors, for example, or Kinesin - tubulin interactions do not appear to be covered by any of the subheads listed under these two headings.

Being able to see tubulin moving into cilia allowed for first insights into how this transport is regulated to make sure cilia will have the correct size.»

In our model, quantum coherence emerges, and is isolated, in brain microtubules until the differences in mass - energy distribution among superpositioned tubulin states reach a threshold related to quantum gravity.

To determine whether SIV infection leads to corneal nerve fiber loss, the researchers immunostained corneas for the nerve fiber marker βIII tubulin.

Its wall is made up of 13 protofilaments, each consisting of heterodimeric subunits containing two related tubulin proteins.

On the other hand, a new tubulin dimer can bind to the end.

In this situation, the microtubules continue to grow until the concentration of tubulins falls below a critical value.

The reason for the rapid development of resistance was that these fungicides were single site inhibitors of fungal microtubule assembly during mitosis, via tubulin - benzimidazole - interactions.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system.

What they didn't know was how tubulin and another cell organelle known as flagella fit into the process.

«Researchers image, measure tubulin transport in cilia.»

The anthelmintic drug mebendazole induces mitotic arrest and apoptosis by depolymerizing tubulin in non-small cell lung cancer cells.

An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.

It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro.

We will use as an example the quantification of tubulin polyglycylation in wild - type testes and in Bug22 mutant testes, which present defects in the deposition of this posttranslational modification.