The plan is to scale up to collecting whole -
tumor genome sequence data from 3,000 to 4,000 patients annually.
Moreover, the ever - growing catalogues of
tumor genome sequences — such as those created by the Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC)-- enable integrated analyses of germline variation with somatic mutation data across tumor types (i.e. pan-cancer analysis).
Not exact matches
It will focus its initial clinical
sequencing efforts on cancer, teaming up with the Moores Cancer Center at the University of California, San Diego to
sequence the
genomes of every patient who is treated there, as well as perform a full
genome sequence on their
tumors.
To determine how the cells switch from one type to another, they took three human uterine carcinosarcoma samples and
sequenced the
genomes of cells in two parts of each
tumor: the carcinoma and sarcoma components.
«Studies like the current one involving rhabdomyosarcoma are giving us a close - up look at the way cancer evolves in response to treatment,» said study co-author Richard K. Wilson, Ph.D., director of The
Genome Institute at Washington University School of Medicine in St. Louis, where scientists have extensive expertise analyzing tumor recurrence using whole - genome seque
Genome Institute at Washington University School of Medicine in St. Louis, where scientists have extensive expertise analyzing
tumor recurrence using whole -
genome seque
genome sequencing.
Sequencing a single
genome yields a terabyte or more of data; Dishman's kidney
tumor yielded 5 terabytes.
The 15 AACR scientists, who were already in Washington, D.C., to meet with the U.S. Food and Drug Administration on genetic testing for cancer patients, tossed around ideas including expanding NCI's
tumor genome -
sequencing efforts and getting the government to cover the costs of genomic
tumor profiling.
The team integrated three, complementary gene
sequencing approaches to look for mutations in
tumor cells from SS patients: whole -
genome sequencing in six subjects,
sequencing of all protein - coding regions (exomes) in 66 subjects, and comparing variation in the number of copies of all genes across the
genome in 80 subjects.
His laboratory develops and deploys new biochemical and computational methods in functional genomics, to elucidate the genetic basis of human disease and human physiology, and to create and deploy novel techniques in next - generation
sequencing and algorithms for
tumor evolution,
genome evolution, DNA and RNA modifications, and
genome / epigenome engineering.
Hadfield and his colleagues are doing large - scale genomic analyses of different
tumor types, as part of the massive Genomics England project to
sequence 100,000 British
genomes.
During the last three years the Spanish Consortium for the Study of the
Genome of the Chronic Lymphatic Leukemia, where the researchers of the present study already collaborate, has sequenced the genome of hundreds of patients with the more common leukemia in our society, identifying new mechanisms of tumor progression and new therapeutic ta
Genome of the Chronic Lymphatic Leukemia, where the researchers of the present study already collaborate, has
sequenced the
genome of hundreds of patients with the more common leukemia in our society, identifying new mechanisms of tumor progression and new therapeutic ta
genome of hundreds of patients with the more common leukemia in our society, identifying new mechanisms of
tumor progression and new therapeutic targets.
Without the
genome sequence of Lacks» healthy cells or that of her original
tumor, it is difficult to trace the origin of these alterations.
Of the several thousand cancer patients treated annually at the Heidelberg National Center for
Tumor Diseases (NCT), whole -
genome sequencing of
tumors is offered to all patients who might benefit, says Eils.
He also plans to leverage the latest
sequencing technologies to understand how complex rearrangements impact the
tumor epigenome and perturb cancer
genome structure over time.
First, iCAGES analyzes the patient's entire
genome, comparing it to the genomic
sequence of the patient's
tumor to identify possible cancer - causing variants.
HM - SNS allows researchers to
sequence the
genomes of single
tumor cells and study multiple cells simultaneously, both lowering the cost and boosting data analysis for such studies.
As the cost of DNA
sequencing continues to plummet, the lab will move ever closer to a once - elusive goal: economically processing every patient's complete
genome in both
tumors and healthy cells.
Unlike previous studies, which tracked lymphoma progression by monitoring the
sequence of just one cancer - associated protein, CAPP - Seq can identify a much larger range of mutations in the
tumor genome.
Future additions to the RM collection may include whole
genomes from persons with Hispanic, African and mixed ancestries, as well as a set containing
sequenced genes of both malignant
tumor and normal cells from the same individual.
To help detect genetic mutations and better understand this disease, a group of researchers at USU and the nationwide Cancer
Genome Atlas Research Network examined 173
tumors, performing six genomic tests, such as DNA and RNA
sequencing.
Sequencing RNA, not just DNA, could help doctors predict how prostate cancer
tumors will respond to treatment, according to research published in the open access journal
Genome Biology.
The Yale - led study performed whole exome
sequencing ¬ — a technique for
sequencing all the expressed genes in a
genome ¬ — on TNBC
tumors to identify mutations in specific genes or pathways that may indicate response or resistance to the standard of care, which is anthracycline / taxane (ACT) chemotherapy.
Almost 10 years to the day after the human
genome sequence was completed, a group of researchers has unveiled the first whole
genome sequences of prostate
tumors.
But by
sequencing the entire
genomes of seven prostate
tumors and comparing them with
genomes from the patients» normal cells, the researchers discovered an unexpected phenomenon.
Sequencing the whole
genome of a prostate
tumor, says co-author Levi Garraway, a physician and biologist at Harvard Medical School in Boston, allowed the researchers to see «biology that would have been invisible with any other method.»
New
sequence of the endangered marsupial's
genome could help future generations fight off devastating facial
tumor disease
In these experiments, Berger and colleagues show that somatic PREX2 mutations identified through whole -
genome sequencing of human melanoma can contribute to enhanced lethality of
tumor xenografts in nude mice (Figure 3B, S6B, and S6C; Berger et al., 2012).
Sequencing the
genomes of
tumor cells has revealed thousands of genetic mutations linked with cancer.
First of all, only the metastatic sample was whole -
genome sequenced — the primary
tumor and matched normal were not.
We developed SomaticSniper to detect somatic mutations in whole -
genome sequencing data while allowing for
tumor contamination of normal cells that occurs in some types of leukemia.
Over the next three years, we will
sequence the
tumor genomes of 600 pediatric cancer cases to characterize the inherited and acquired genetic changes that underlie cancer in children.
There are many good reasons for this, but with low - cost whole
genome sequencing I think that we can explore the whole
genomes of rare
tumor types as well.
Zhang et al performed whole -
genome sequencing on
tumor samples and matched normals from 12 children with ETP - ALL.
HLI and UC San Diego have signed a collaborative research agreement to develop protocols and procedures to enable whole
genome, microbiome and
tumor sequencing and analysis of consenting UC San Diego research patients.
For this compilation, I've emphasized publications in which whole -
genome or exome
sequencing was employed to multiple
tumors or cancer cell lines, with the goal of identifying significantly altered genes or pathways in malignant cells.
Thanks to the Segals» gift, the pancreatic - cancer effort will
sequence the
genomes of
tumors from up to 225 patients from the University of Chicago Medicine - NorthShore University HealthSystem pancreatic cancer program over the next three years.
In the very near future, it may become feasible and cost - effective to perform whole -
genome sequencing (WGS) on hundreds or thousands of
tumors of a certain type to exhaustively identify recurrently mutated genes.
By
sequencing the complete
genomes of primary
tumor, relapsed
tumor, and matched normal (skin) samples from 8 AML patients, our group was able to study clonal evolution of AML
tumors at the genetic level.
Also this week, members of the St. Jude Children's Research Hospital — Washington University Pediatric Cancer
Genome Project (PCGP) described the whole - genome sequencing pediatric diffuse intrinsic pontine glioma (DIPG), 7 cases with tumor and matched germlin
Genome Project (PCGP) described the whole -
genome sequencing pediatric diffuse intrinsic pontine glioma (DIPG), 7 cases with tumor and matched germlin
genome sequencing pediatric diffuse intrinsic pontine glioma (DIPG), 7 cases with
tumor and matched germline DNA.
We published the first complete
genome of a
tumor in 2008 and have applied next - gen
sequencing technologies to breast cancer, glioblastoma, ovarian cancer, leukemia, and other cancers.
Some of us have been preaching whole -
genome sequencing for
tumor genomes for a long time.
Here, we
sequenced the
tumor and non-
tumor genomes (> 80X coverage) and transcriptomes of four HCC patients and identified 255 HBV integration sites.
Whether identifying rare subclones missed by standard bulk
sequencing, or identifying co-mutation patterns in subclones, Tapestri Single - Cell DNA Panels can be applied across a wide number of applications, including hematologic malignancies, solid
tumor profiling, and
genome editing programs.
This public
genome repository comprises
genome results from both our Standard
Sequencing Service (69 standard, non-diseased samples) and the Cancer
Sequencing Service (two matched
tumor and normal sample pairs).
«Multiclonal invasion identified in early breast
tumors by topographic single cell
genome sequencing»
As part of the Cancer
Genome Atlas Project (TCGA), we've been applying both targeted (capture - based) and whole - genome sequencing approaches to tumor samples and matched normal con
Genome Atlas Project (TCGA), we've been applying both targeted (capture - based) and whole -
genome sequencing approaches to tumor samples and matched normal con
genome sequencing approaches to
tumor samples and matched normal controls.
Exome
sequencing alone detected driver gene alterations in 26 % of
tumors, compared to 72 % by
genome sequencing.
In this study, we utilized patterns of mutagenesis known as mutational signatures, which are imprints of the mutagenic processes associated with MMR deficiency, to identify MMR - deficient breast
tumors from a whole -
genome sequencing dataset comprising a cohort of 640 patients.
Using assembly - based whole -
genome DNA
sequencing, we found previously undefined genomic rearrangements in human rhabdoid
tumors.
In it, the authors review bioinformatics approaches for evaluating single - base substitutions in both coding and noncoding regions, with an emphasis on large cancer resequencing efforts including the
Tumor Sequencing Project, the Cancer
Genome Atlas (TCGA), and the AML1 cancer g
Genome Atlas (TCGA), and the AML1 cancer
genomegenome.