I am also involved in making various
types of amplicon as well as whole - genome shot gun libraries to sequence on different illumina platforms (MiSeq, NextSeq, HiSeq) and PacBio to study the relationship between microbiome and several diseases.
Here, we employed cDNA
amplicon sequencing using a long - read portable sequencer, MinION, to characterize various
types of mutations in cancer - related genes, namely, EGFR, KRAS, NRAS and NF1.