Roberta spent days, using tiny pliers and a glass knife,
slicing ultrathin layers of the fly's internal organs and preparing them to view through an electron microscope.
Using electron microscopy to look at thousands of
ultrathin brain
slices taken from awake and sleeping mice, they found that after sleep, the size of most synapses — specifically, the surface area where two neurons touch each other — shrank by about 18 percent.