Therefore, we used microarray analysis to perform pairwise comparisons of the transcriptional profile of EFTF - expressing pluripotent cells to three regions of the Xenopus embryo: the eye field (EF) including
underlying mesendoderm, posterior neural plate (PNP) and the non-neural, flank, lateral
endoderm (LE, Figure 1A).
Collectively, these findings augment the
endoderm GRN and reveal a panel of target genes
underlying the Ta, Tbx16, and Mixl1 mutant phenotypes.