Sentences with phrase «unlabeled h3»

G9a (4 µM in 20 mM Tris pH 8.0, 250 mM NaCl, 500 µM SAH, and 0.01 % Triton) was incubated with 40 nM F - H3 (1 - 15) peptide, and different concentrations of BIX - 01294 (purchased from Sigma - Aldrich), UNC0224, UNC0638 and unlabeled H3 (1 - 25) peptide from 0.1 to 100 µM were added.