Sentences with phrase «unsupervised hierarchical cluster»

Proteins (n = 59) that were quantified and determined to be significantly different in abundance by ANOVA (p ≤ 0.01) when comparing CFS from nPTLS subject samples and allow for separation of these two syndromes when performing unsupervised hierarchical cluster analysis.

Unsupervised hierarchical clustering and PCA were employed to determine if the observed quantitative differences in protein abundances were sufficient to distinguish these two patient groups (this was de facto blinded — as samples were run in a random order and uncoded as to disease group afterwards).

The proteins considered in the unsupervised hierarchical clustering analysis were quantified in individual samples and found to be significantly different in abundance by analysis of variance (ANOVA p ≤ 0.01, Table S3); while PCA analysis considered all proteins quantified in each individual sample.

Unsupervised hierarchical clustering of the 5,959 genes, using 100 random permutations, gave rise to 115 groups, containing a total number of 2,855 transcripts, with a probability of 95 % or higher that clustering was not random (Supplemental File S2).