Sentences with phrase «urodele myotube»

This was done using shRNA technology specific for BRCA1 in human myotubes (skeletal muscle fiber cells).

Within days, the neural stem cells began to make proteins typical of muscle cells, and even joined with the muscle cells to form myotubes, tubes of fused cells that make up the bulk of living muscle.

In vitro experiments in myogenic cells initially suggested that IL - 15 was an anabolic factor for skeletal muscle, able to stimulate the accumulation of contractile protein in differentiated myotubes (8 — 10).

Post-transcriptional regulation of autophagy in C2C12 myotubes following starvation and nutrient restoration.

Philipp Weissert (Tanaka, MPG)-- «Bmp proteins in urodele myotube cell cycle re - entry and in regeneration» (2008)

Myotubes were precultured in increasing insulin concentrations for 4 days and subsequently stimulated acutely by insulin.

However, further investigations are needed to clarify whether previous metabolic influences at the level of quiescent satellite cells in vivo can induce irreversible metabolic changes in cultured human myotubes.

In this context, cultures of primary human myotubes offer excellent material for performing studies under standardized conditions.

Myotubes established from type 2 diabetic subjects express a reduced GS mRNA and protein compared with control subjects (5).

Satellite cells are quiescent cells that have to be activated in vivo before proliferating and differentiating into myotubes.

Preculturing myotubes for 4 days at increasing insulin concentrations did not change the basal FV0.1 in diabetic or control cultures (Figs. 3A and B).

Our dose - response curve at chronic high insulin levels allowed us to differentiate between a primary defect (probably genetic) and the induction of secondary insulin resistance in myotube cultures due to hyperinsulinemia.

B: The GS activities measured at 10.0 mmol / l in the basal state and in the stimulated state in myotubes precultured at the indicated insulin concentration.

Moreover, the satellite cells were subsequently allowed to differentiate to myotubes, which changed their protein expression and metabolism.

Consistent with these findings, satellite cells from SMA mice differentiate prematurely both in vivo and in vitro and form fewer myotubes.

Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications.

Survival motor neuron protein deficiency impairs myotube formation by altering myogenic gene expression and focal adhesion dynamics.

Our «biased» profiling is based on an in vitro model of mouse terminally differentiated myotubes, induced to re-enter the cell cycle by the E1A oncogene (23).

Along those lines, it was also recently shown that ROS function as important signaling molecules for muscle hypertrophy in vitro, where it was found that IGF - 1 induced hypertrophy of myotubes in culture is suppressed by antioxidants (15).

Overexpression of FOXO3A in mouse myotubes also significantly up - regulates MAFbx expression (49).

Amino acids and insulin act additively to regulate components of the ubiquitin - proteasome pathway in C2C12 myotubes