Sentences with phrase «use a restriction site»

For example, if you must use a restriction site that will be blocked by Dam or Dcm methylation, you can ensure this site remains unblocked by first cloning your DNA into a dam — / dcm — strain of E. coli such as JM110 and re-purifying it.

Not exact matches

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If Facebook and Google crack down on «fake news» sites, the Microsoft researcher argues, those who have an interest in generating that kind of content will find ways around the restrictions, as many already have by using visual «memes» instead of links to news stories.

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Some of the medical studies I used as a reference are: Caloric restriction and intermittent fasting: Two potential diets for successful brain aging and Dietary Factors, Hormesis and Health, found on the US National Library of Medicine Site, Cardioprotection by Intermittent Fasting in Rats on the American Heart Association Site and Effect of Ramadan intermittent fasting on aerobic and anaerobic performance and perception of fatigue in male elite judo athletes from the Journal of Strength and conditioning research.

We've noted before that, due to archaic House rules, Members of Congress are prohibited from posting videos to YouTube (the use of YouTube is seen as de facto advertising for it, and restrictions also rule out posting on a site with advertising).

Restriction digest assay (RFLP)- if your targeting strategy can be designed in such a way that a restriction site is lost or gained, this can be used to screen for targeRestriction digest assay (RFLP)- if your targeting strategy can be designed in such a way that a restriction site is lost or gained, this can be used to screen for targerestriction site is lost or gained, this can be used to screen for targeted clones.

Relevant restriction sites used for cloning or for Southern blot analysis are indicated.

There are hundreds of restriction enzyme families but few are used to date, because no tool is available for the systematic evaluation of restriction enzyme combinations that can enrich for certain sites of interest in a genome.

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But, you need to upgrade to become a member, to be able to send messages and to use the site completely without any restrictions.

And no restrictions (within reason) and full use of the site makes that easy.

Unlike many other adult dating sites, there are no location restrictions placed upon your matches when you use our site.

The most effective free sugar daddy sites evaluations are organized right into top listing based on numerous requirements, including restrictions, performance, functions and their recognition, affordability and simplicity of use.

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We use morphometrics, allozyme electrophoresis, mitochondrial DNA (mtDNA) restriction - site analysis, and analysis of hypervariable minisatellite DNA to measure variability within and distances among island fox populations.

Because of this, you can book travel using whatever travel site you want, with no blackout dates and no seat restrictions.

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In the case of CS: GO, the trading scene got particularly sketchy when gambling sites that allowed people to bet using items began to pop up, forcing Valve to tighten trading restrictions.

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Welcome to the limelight, curated by Natalie Woyzbun, includes works by Jessica Craig - Martin, Instant Coffee, Christian Jankowski, David Kramer, Liisa Lounila, and Tony Matelli that invite the viewer into spaces of entertainment and leisure; You don't live here anymore, curated by Montserrat Albores Gleason, features works in which ideas of dwelling and building transform the site of art and its methods of construction; Uninvited (working with restrictions), curated by Kerryn Greenberg, considers how success can be realized in failure, and freedom found through restriction, in the performances of Steven Cohen and his partner, Elu; and In Other Words, curated by Mariangela Méndez Prencke, focuses on bilingual works that use collage and other visual devices to translate themselves into a foreign context.

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Recently, legal topics surfacing on Tom's site have included land use issues («The Aesthetic Value of Vineyard Restrictions»), international trade protectionism in Spain («Screw Caps Banned!»)

The distinction between commercial use seems fine: if a company buys a drone for its own internal site mapping or internal operations, providing it meets the other restrictions, it does not need permission.

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Facebook is releasing a new version of Messenger, its standalone messaging app, specifically for users under the age of 13 — those who are too young to use Facebook due to the site's age restrictions.

According to TorrentFreak, however, many free users are using rigged Spotify installation files freely available on many sites to get around those restrictions.

For Internet Explorer, you can easily use Internet Options in Control Panel to add restrictions and prohibit sites by content or otherwise.

Use this first strategy with one condition; for the social networks where you spend most of your social networking time (like I currently do with Twitter), use an image tailored to that site's guidelines and / or restrictions, which is where my recommendations come frUse this first strategy with one condition; for the social networks where you spend most of your social networking time (like I currently do with Twitter), use an image tailored to that site's guidelines and / or restrictions, which is where my recommendations come fruse an image tailored to that site's guidelines and / or restrictions, which is where my recommendations come from.

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All of these apps have the same age restrictions as the sites they are used with and, as such are not intended for use by people under the age of 13.

«Net neutrality» requires that broadband networks be free of restrictions on content, sites, or platforms; they should not restrict the equipment that may be used with them, nor the modes of communication allowed on them.

Even if vendors are able to offer MLSs and brokerages a better arsenal of tools to attract consumers to their sites, some worry they won't be able to make use of them if NAR doesn't relax restrictions in its model IDX policy that govern what content can appear around listings.

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