Sentences with phrase «use aliquots»

For long - term storage, viral preps should be divided into single - use aliquots, and stored at -80 °C.

Not exact matches

Two aliquots each of purified plasmid DNA's containing envelope sequence subtype refrenceThe plasmids in this kit are for use in HMA only.

Western blots were performed using 20 µg aliquots of protein from fetal or adult hearts, resolved on 10 % Tris - glycine - SDS polyacrylamide gels (Bio-Rad, Hertfordshire, UK) and immobilised on PVDF membranes.

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

An aliquot of coded plasma from the CFS and healthy controls was tested at RKI by an ELISA using recombinant Gag and Env proteins used recently to investigate XMRV infection in German prostate cancer patients [14].

2 µl aliquots of the purified PCR product were used for sequencing PCR with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).

A 100 - μl aliquot of the buffer was used for the lipase assay in combination with 100 μl of a 10 % Intralipid / [3H] triolein emulsion for 1 hour at 25 °C.

These aliquots were used to initiate blood infections in naïve mice that were then used as donors of asexual parasites to mice involved in the posttransmission experiments.

(a) As a laboratory, you must use the invalid test result criteria for the initial and confirmation testing as required by the HHS Mandatory Guidelines and you must use two separate aliquots — one for the initial test and another for the confirmation test.