Sentences with phrase «use cultured cells»

Exactly how the genetic risk factors contribute to the disease is not readily apparent in most cases, and requires cellular and molecular biology investigations that use cultured cells and mouse models.

Years ago W.U.'s Holtzman and former colleague Marc Diamond, who has since moved to the University of Texas Southwestern Medical Center, identified tau - binding antibodies that slow the spread of toxic tau clusters in a lab assay using cultured cells.

Furthermore, using cultured cells, they examined how the sex - related genes were regulated.

Using cultured cells derived from human tumors of the breast and prostate gland, they confirmed that the IL6R / STAT3 / miR -34 a feedback loop is also activated in other tumor types.

Not exact matches

One habit of Prince's they told me about struck me as so counter to our times that I came to see it as illustrating what set him apart from today's culture of celebrity: he allowed no one near him to use a cell phone.

Bethencourt and Shigeta figured they could use cell cultures to grow meat without slaughtering any cows or chickens — but why rely on those animals at all?

In my cell biology course, we investigate the biology and chemistry of a cell surface receptor that helps induce good feelings in us when it binds to a chemical compound found in incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and rituals.

While some still object to cells being taken from animals and used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.

3M Delivers Early Purification with New Hybrid Purifiers, Single - use functionalised media provides scalable purification performance in cell culture

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

While other papers have examined these mutations using expensive and time - consuming experiments on live ferrets and laboratory cell cultures, Deem and Melia Bonomo used the pEpitope method to rapidly calculate how much the egg - passage mutations would decrease vaccine efficacy in humans.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Researchers from Duke University had previously used CRISPR to correct genetic mutations in cultured cells from Duchenne patients, and other labs had corrected genes in single - cell embryos in a laboratory environment.

In the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implant.

This study identifies myomerger as a fundmentally required protein for muscle development using cell culture and laboratory mouse models.

Bioengineers at the University of California, San Diego have proven that when it comes to guiding stem cells into a specific cell type, the stiffness of the extracellular matrix used to culture them really does matter.

Despite being trapped and baked within a polymer, cell staining shows that the cultured cells remained alive, says Fernandez, an obvious necessity if they are to be used for artificial tissues.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

Protein expression in these glioblastoma cells more closely mimicked that in real cancer cells than in 2D cultures of cells, indicating that this method could be used to study cancer (Nature Nanotechnology, DOI: 10.1038 / nnano.2010.23).

Using human fetal «mini-brains» grown in 3 - D cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

Dr. Zubair already grows such cells in his Mayo Clinic laboratory using a large tissue culture and several incubators — but only at a snail's pace.

They then used fluorescent antibodies that bind specifically to the protein to trace the virus's interactions with various kinds of cultured cells.

The cells used to build the organs could be cultured from stem cells taken from fat or bone - marrow tissue, he says: «We could engineer a blood vessel from your own cells.»

Although the bulk of the commercial manufacturing uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is using goats; PPL Therapeutics, which is using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.

Droplet - based microfluidics is a tool used in various applications like cell cultures, chemical synthesis and DNA sequencing.

Often times we'll use an animal model or cell culture model to test a bioactive to see if it has efficacy.

The students used a 3 - D printer to design a smaller and more cost - efficient bioreactor — a miniaturized culture device where neural stems cells are grown and eventually become small laboratory brains for research.

The team used human embryonic stem cells — which can transform into any cell of the body — and cultured them in a mixture of chemicals to grow human brain cells.

The study team removed fibroblasts (skin cells) from DBA patients, and in cell cultures, using proteins called transcription factors, reprogrammed the cells into iPSCs.

When we first posted this story, we used the headline: «Stem cell scientists reveal «unethical» work culture».

Currently, most influenza vaccines in the United States are produced using chicken eggs, while a few are made in cell culture or by using recombinant DNA technologies.

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.

The researchers then used a 3 - D cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor cells and finally the proximal tubule cells found in kidney nephrons.

Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine used a powerful statistical tool called «design of experiments» or DOE to determine the optimal cell culture formula to grow and produce hPSCs.

Cellular toxicity was unrelated to the type of «carrier» solution used in the cell cultures, the use of HES made from different origins (potato versus corn starch), or the time cells spent in culture with HES.

Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been using cultured mesenchymal stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.

Human chondrosarcoma HCS - 2 / 8 cells, which are a type of benign bone cancer cells, can be used to investigate how optimization of culture conditions could improve the synthesis of cartilage - specific molecules.»

The new research, which studied the immortalization process using genome - engineered cells in culture and also tracked skin cells as they progressed from a mole into a malignant melanoma, suggests that telomerase plays a more complex role in cancer.

They might be put to use right away to track cultures of immune cells as they migrate in response to chemical stimuli.

The cells will enable researchers to use lab - grown cultures to study how the diseases develop.

«What made the difference are our unique cell culture conditions and 3 - D matrix, which allowed cells to grow and develop much faster and longer than the 2 - D culture approaches that are more typically used.»

Using a labor - intensive cell culturing technique for hunting retroviruses that he had pioneered in the 1970s, Ruscetti had transmitted the pathogen from patients» T - cells to uninfected T - cells in the laboratory.

To unravel the complex journey of the body's GPCRs, scientists used biophysical tools, including fluorescent biosensors; biochemical tools, such as cell signaling in neuronal cultures; as well as computational models.

Hsiao and colleagues carefully gathered skin samples from donors, and then grew the skin cells in culture before converting them into iPS cells using the methods created by Yamanaka.

We were able to use pharmaceuticals to induce cell self - eating effects and stimulate dedifferentiation though not as effectively as 3D culture, so we need to do more work on this.

After the pig experiment worked well, he used his Boston area facility to culture the 30 million cardiac muscle cells that he says were injected into a heart disease patient in mid-2011.

Cultured human cells are a better alternative and are already used by one manufacturer, says the report.

In animal and cell culture studies, the drug inhibited growth both in estrogen - dependent breast cancer cells and in cells that had developed resistance to the anti-estrogen tamoxifen and / or to the aromatase inhibitors, two of the most widely used types of drugs to prevent and treat estrogen - dependent breast cancer.

The journal's home page explains that translational medicine «builds on basic research advances — studies of biological processes using cell cultures, for example, or animal models — and uses them to develop new therapies or medical procedures.»