Sentences with phrase «use different assays»

It should be noted that labs use different assays and methodologies to measure free testosterone.

In a second experiment, they used a different assay to look at an additional 6000 genes.

Not exact matches

Diagnostic assays for on - farm use have different constraints compared with those used in traditional laboratory environment

The researchers measured toxicity using a biological assay, by counting the number of dead Daphnia — tiny crustaceans — in water containing the different ladybird toxins.

To determine the effects of the loss of IL - 15Rα on exercise performance in vivo, we quantified activity of IL - 15Rα — KO mice and B6129 control mice using two different assays: cage wheel running and quantification of photobeam breaks.

Once developed, the assays will be used to analyse in vitro cytokine and chemokine induction, e.g. to compare different versions of a vaccine, or the same vaccine produced in different host cells, or different batches of a vaccine to demonstrate batch - to - batch reproducibility.

We will extend our HTS tests using different cell assays and extended chemical components libraries.

This will include testing the mechanism of action of different adjuvant and antigen formulations by measuring B - and T - cell responses and by using various functional assays (ELISA, FACS, cytokine profiles), transcriptomics and / or proteomics.

Expression profiles can also be determined following relevant antigenic in vitro stimulation using whole blood or PBMC - based assays with samples derived from individuals / patients collected at different time points post vaccination / immunisation.

After 24 h of irradiation, amount of cell death in different groups was measured using TUNEL assay (EMD Millipore, Cat.

cDNAs prepared from 45 adjacent normal - tumor paired hepatoma samples were provided by different sources (30, 31) and used in quantitative RT - PCR assay.

Overall a total of 1969 pools of mosquitoes collected at different sites in Kedougou between May to December 2011 were tested using the ZIKV rRT - PCR assay.

iTeos uses a platform of in vitro and ex vivo screening assays to reproduce immunosuppression induced by different components of the tumor microenvironment.

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

Testing of 51 specimens from CFS patients and 56 matched and healthy controls from the US was performed independently in three laboratories for XMRV DNA by using several PCR tests and for anti-XMRV antibodies using different serological assays.

(A) EC50 values from MTT assay using silver nanoparticles with different coatings and HepG2 cells; (B) Analogous with Neuro - 2 cells; (C) Detection of oxidative stress from Ag - NPs (concentration 5 pM) via formation of protein carbonyls incubated with HepG2 cells.

Additional experiments using RNA isolated from stocks of viruses from different virus families showed no cross reactivity of the assay with influenza A virus, influenza B virus, rhinovirus, human coronaviruses 229E, OC43, and NL63, and adenovirus (data not shown).

All assays were inoculated with actively growing mycelium and replicated twice at different times using two replicates each time.

Assay, sample and side - to - side variation were quantified by analysis of variance and presumptively incorrect decisions related to high - risk disease were determined using the result from each MRD assay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatAssay, sample and side - to - side variation were quantified by analysis of variance and presumptively incorrect decisions related to high - risk disease were determined using the result from each MRD assay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatassay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatment.

This is the first study demonstrating smoking - induced expression changes for this particular set of genes and importantly, it is the first time partial reversibility (MUC5AC) and irreversibility (GSK3B) and has been demonstrated using two different cohorts of samples with two independent assays for expression quantification.

Prior research had developed a number of new compounds making use of a novel drug discovery paradigm which begins with natural products extracted from plants; it then entails selecting synthetic derivatives which demonstrate efficacy in multiple assays testing protection against different factors of the nerve cell damage and death which take place in brain injuries and in age - associated neurodegenerative conditions.

These lipases of different cellular origins share a common function and thus can not be differentiated by use of a catalytic assay such as the ones that are being used to determine lipase activity in serum.

The researchers measured toxicity using a biological assay and found that five common ladybug species each have different levels of toxic defense.

(The DOE also is apparently considering a different fast reactor design that would use high - assay, low - enriched uranium fuel, but this material is in short supply and a new production source would have to be established.

The Quick Start Guide explains the different areas of medical / research laboratory work (Histology, Phlebotomy, Hematology, Microbiology / Bacteriology, Chemistry, Blood Bank, and Molecular) and how the tests (different assays that are used to determine if disease is present, whether it is responding to medication and even, what medications will be most effective) and products (the analyzers, the reagents, and the other tangible items that are used to provide the end result to the attending physician) available fit within those areas.