Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and
we use electroporation to ensure a good transformation efficiency.
Using electroporation — in Corn's words, «we run an electric current and make holes in the cells» — he'll inject the complex and replacement parts through the cells» protective membrane.
Not exact matches
Using Pulse Electric Field Processing (PEF) the patent protected E-FLO
Electroporation System provides significant product improvement in chip crunch, taste and texture as well as reduction in acrylamide formation and oil content.
«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized
electroporation of mammalian cells by
using electrostatic manipulation in a water - in - oil droplet.»
The first authors, Assistant Professor Hirofumi Kurita and Professor Akio Mizuno, said, «W / O droplet
electroporation is operated
using a DC power supply, which obviates the need for an expensive pulse generator.
However, most commercial
electroporation - based transfection methods require the
use of specialized pulse generators to produce short electrical pulses at high voltage.
There have been improvements in W / O - droplet
electroporation electrodes for
use in disposable 96 - well plates for concurrent performance (Fig. 2).
In addition, the size of the droplet is quite small compared with that in conventional bulk
electroporation, resulting in the
use of fewer materials in high - throughput analysis.»
They then
used a method known as
electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.
For both BayGenomics parental ES cell line E14Tg2a.4 (also
used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple
electroporations and germline transmission are available.
INO - 3112 is injected into a muscle followed by
electroporation, a technique that
uses controlled, millisecond electrical pulses that make it easier for the DNA plasmids to enter muscle cells.
Whether a protocol
uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after
electroporation, optimized culture conditions are key to maintaining cell viability and biological function.
Cell culture steps were
used by both groups to let cells recover after thawing and / or
electroporation, and additionally to generate erythroid progenitor cells from edited CD34 + cells [1].
Electroporation of cultured cells was carried out
using the Neon Transfection System (Invitrogen).
For a typical library, Addgene usually does four to eight
electroporation reactions
using a total of ~ 400ng of library DNA.
In this study we review the most commonly
used indel detection methods and
using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly
used CRISPR / Cas9 delivery formats, including lentivirus transduction, plasmid lipofection, and ribo nuclear protein
electroporation, on the dynamics of indel profile formation.
Additionally, the authors tested for cellular specificity by
using as a convenient experimental framework in utero
electroporation of a plasmid that allowed co-expression of the PLE enzyme and of a fluorescent tag (mCherry).