Use fluorescent markers as a guide for blot - film orientation.
The most common probes, which
use fluorescent markers or radioactive labels to pinpoint the virus's location in a tissue sample, sometimes have difficulty distinguishing the target — HIV RNA and DNA — from surrounding cellular components.
After watching those mice thrive for several months, Lagasse repeated the experiment
using fluorescent markers to trace the path of the liver cells.
Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.
And by
using fluorescent markers to tag these cells, they have even been able to detect them by using a simple mobile phone camera.
Now, researchers at the University of Cambridge have
used fluorescent markers to track cell development in the embryo of a cartilaginous fish — a little skate in this case — and found that these thorny scales are in fact created from the same type of cells as teeth: neural crest cells.
«It tells you about the ecosystem of the tumour», Simona says, «
using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.
He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.
STAR uses advanced computer vision tech that
uses fluorescent markers and 3D cameras to get a detailed view of the surgical site.
Not exact matches
The lab tracked the differentiating process
using fluorescent cell imaging, which tested for the necessary
markers during each step.
Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and
use the included software to sort them by purely visual characteristics such as shape and size,
fluorescent markers, or both.
Kelley and his team also
used mice with
fluorescent markers in different cells of the ear followed by next generation sequencing.
Using new technologies for labeling cellular machinery with light - activated
fluorescent markers, they could «turn on» just one molecule at a time.
Fluorescent dyes, which are frequently
used as
markers in biological experiments, are particularly hard to detect in Raman spectroscopy because the fluorescence tends to wash out the signal.
Scientists at The Institute of Cancer Research (ICR)
used the cells of fruit flies, modified with a
fluorescent marker that is activated when the cells are put under stress, to identify the signals responsible for driving up activity of the ER.
Using L1 tagged with a
fluorescent marker, the group observed higher numbers of
fluorescent iPSCs from non-human primates compared with humans.
It
uses a specially developed
fluorescent marker which attaches to cell proteins when injected into patients.
«Here we have demonstrated the further
use of this novel technology to develop highly sought after biologically relevant
fluorescent markers quickly and easily for different imaging needs,» says Caldwell, who led the study.
So Gaddis
used mice in which regulatory T cells would be tagged with two
fluorescent markers.
Researchers
use these receptors — or
markers — to identify and isolate adult stem cells by «tagging» the chemical messages that bind to those specific receptors on the stem cell with
fluorescent molecules.
Pharmacology Assays
Fluorescent or luminescent
markers are also
used in pharmacological and drug discovery assays.
Fluorescence - based assays
Fluorescent markers such as EGFP, YFP, mCherry and mTomato or fluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and t
Fluorescent markers such as EGFP, YFP, mCherry and mTomato or
fluorescent biosensors can be used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and t
fluorescent biosensors can be
used to measure a variety of real - time cell - based activities, including, intracellular transport, protein signaling, receptor desensitization, migration, division, apoptosis, metabolism, differentiation, chemotaxis, transcription and translation.
A gene for green
fluorescent protein from the jellyfish, Aequorea Victoria, has been inserted into the cats» DNA and is
used as a
marker for cells carrying a second gene which confers resistance to the feline version of the AIDS virus, the Feline Immunodeficiency Virus (FIV).