Sentences with phrase «use in gene expression»

Real - time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven use in gene expression analysis at both low and medium throughput.

Not exact matches

The recent Lancet Breastfeeding Series refers to new discoveries being made about breastmilk — such as its role in gene expression — so it can be expected that Nestle will use the strategy of launching «new improved» formulas that are «closer to breastmilk» for decades to come (Nestle is currently investing heavily in epigentics research).

A new study in Biological Psychiatry found that heroin use is associated with excessive histone acetylation, an epigenetic process that regulates gene expression.

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

Scientists at Southern Methodist University, led by Professor and Chair of Biological Sciences Santosh D'Mello, have used RNA - Seq to conduct transcriptome profiling of gene expression changes in dying neurons.

In the new research, the UB scientists found they could reverse those social deficits with a very low dose of romidepsin, which, they found, restores gene expression and function using an epigenetic mechanism, where gene changes are caused by influences other than DNA sequences.

In one of her experiments she studied gene expression in nematodes subjected to 15 - G pressures, using Ames's human centrifugIn one of her experiments she studied gene expression in nematodes subjected to 15 - G pressures, using Ames's human centrifugin nematodes subjected to 15 - G pressures, using Ames's human centrifuge.

«Autism's social deficits are reversed by an anti-cancer drug: Using an epigenetic mechanism, romidepsin restored gene expression and alleviated social deficits in animal models of autism.»

Gene expression is the process whereby the genetic information of DNA is used to manufacture functional products, such as proteins, which have numerous different functions in living organisms.

PATTERNS of gene expression in a woman's blood may give a clue as to whether she is likely to get pregnant using IVF.

Prasher and Chalfie were awarded a patent for the use of GFP as a marker of gene expression; in total, it earned Prasher just a few hundred thousand dollars in royalties over 15 years.

«Controlling RNA in living cells: Modular, programmable proteins can be used to track or manipulate gene expression.»

Traditional strain - selection techniques are supplemented with strain screening, which can involve examining gene - expression patterns through PCR or understanding global gene expression in relation to environmental factors using gene chips.

Using RNA sequencing, the researchers found multiple genes whose abnormal expression could lead to the high rate of death for cloned embryos, including failure to implant in the uterus and failure to develop a normal placenta.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

To identify genes involved in the patterning of adult structures, Gal4 - UAS (upstream activating site) technology was used to visualize patterns of gene expression directly in living flies.

«The use of a radiofrequency - driven magnetic field is a big advance in remote gene expression because it is non-invasive and easily adaptable,» says Dordick, who is Howard P. Isermann Professor of Chemical and Biological Engineering and vice president of research at Rensselaer.

The second duplication appears in supplemental figure S6, where a scatterplot purporting to show gene expression similarities between the cell lines was used twice.

«For several years the potential for the use of gene expression data in research and clinical applications has been underappreciated due to the inconsistency of the data coming from the various types of equipment.

Together, the two proteins act as a nano - machine that can be used to trigger gene expression in cells.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

The researchers then compared the expression of all genes in six parts of the brain of the two bird species using state - of - the - art molecular techniques, including next - generation sequencing — the first time these tools have been used to find brain properties related to innovation and problem - solving in wild birds.

Another version, the CTC - iChip, rapidly isolates CTCs in a way that does not rely on preidentified tumor antigens, allowing capture of cells with gene expression patterns that may be missed by the antibodies used in the HBCTC - Chip.

Now, using computer simulations, James Carothers and his colleagues at JBEI have sped up the RNA design phase, creating computer tools that will help researchers design molecules to precisely control gene expression in bacteria to optimize fuel production.

This research tool can be used to better understand the function of histone proteins, which play critical roles in the regulation of gene expression in animals and plants.

Gene expression biomarkers are being successfully used to guide therapy decisions in the field of cancer.

To develop a comprehensive view of astrocyte gene expression, the duo used the technique in four very different areas of the mouse brain: two regions of the cortex and the hypothalamus and cerebellum.

Using an innovative crystallization technique for studying three - dimensional structures of gene transcription machinery, an international team of researchers, led by scientists at Penn State, has revealed new insights into the long debated action of the «magic spot» — a molecule that controls gene expression in Eschericahia coli and many other bacteria when the bacteria are stressed.

Using breast cancer patient data taken from The Cancer Genome Atlas, molecular biologists Curt M. Horvath and Robert W. Tell used powerful computational and bioinformatics techniques to detect patterns of gene expression in two cancer subtypes.

The researchers in the RNA Biochemistry Group at Freie Universität Berlin were able to show that mice use this time - of - the - day - dependent change in body temperature to adapt gene expression to clock - dependent requirements.

They then used next generation sequencing — a state - of - the - art method to rapidly measure gene expression — to sequence and quantify the thousands of genes that are expressed in hair cells, in comparison with other cells in the ear.

L2 plays an important role in cells by activating mRNA decay, a system cells use to turn off gene expression when the proteins encoded by the mRNAs are no longer needed.

Using Epiviz and Bioconductor, the research team found consistent regions of DNA methylation changes in colon cancer samples generated by the public Cancer Genome Atlas project and similar gene expression in these regions of DNA methylation changes in other cancer types.

By measuring gene expression in the transgenic mice using DNA microarrays, researchers discovered 50 biomarkers of gene expression that each indicated declining kidney function.

In the Nature Methods paper, Corrada Bravo, UMD computer science doctoral student Florin Chelaru, and undergraduate research assistants from Williams College in Mass. and Washington University in St. Louis used Epiviz to visualize and analyze DNA methylation and gene expression data in colon canceIn the Nature Methods paper, Corrada Bravo, UMD computer science doctoral student Florin Chelaru, and undergraduate research assistants from Williams College in Mass. and Washington University in St. Louis used Epiviz to visualize and analyze DNA methylation and gene expression data in colon cancein Mass. and Washington University in St. Louis used Epiviz to visualize and analyze DNA methylation and gene expression data in colon cancein St. Louis used Epiviz to visualize and analyze DNA methylation and gene expression data in colon cancein colon cancer.

Epiviz implements multiple visualization methods for location - based data (such as genomic regions of interest) and feature - based data (such as gene expression), using interactive data visualization techniques not available in web - based genome browsers.

Using a fluorescent protein to detect Rgs16 expression, the investigators found that this gene is induced by pancreatic tumor formation starting from its earliest manifestation as ductal neoplasm all the way to advanced solid tumor in a spatially and temporally coincidental manner.

While previous investigations into the protein's effects have used either mice in which gene expression was knocked out or transgenic animals that expressed human gene variants throughout their lifetimes, the MGH - MIND - led study used a different approach to investigate the effects of introducing the variant forms of the protein into brains in which plaque formation had already begun.

In the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalIn the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalin Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signalin mammalian cell cultures and were able to regulate intracellular protein levels using light signals.

The new research focused on just nine genes, those most strongly associated with autism in recent sequencing studies, and investigated their effects using precise maps of gene expression during human brain development.

«Using a technique developed by our collaborators at the University of Iowa, we were able to get long - term expression of these human gene variants in the fluid that bathes the entire brain,» says Bradley Hyman, MD, PhD, of the MassGeneral Institute for Neurodegenerative Disease (MGH - MIND), senior author of the report in the Nov. 20 Science Translational Medicine.

The researchers received another surprise when they used recently developed genomic approaches to compare the expression of genes related to calcification in coccolithophores grown under current and future seawater conditions.

However, to make use of that information required an understanding of gene expression and regulation, areas in which institute members made a conscious decision to invest and that became its strengths.

«Telomerase is a unique protein - RNA complex where the protein subunit uses its RNA component as a template to add identical fragments of DNA to the end of chromosomes,» said Emmanuel Skordalakes, Ph.D., associate professor in the Gene Expression and Regulation program of Wistar's NCI - designated Cancer Center.

Using genome - wide approaches, they found that the Rev - Erb proteins repressed gene expression in macrophages primarily by binding to enhancers.

Using clinical, genetic, and gene expression data as filters to distinguish genes whose copy number alteration causes cancer from those for whom copy number changes are incidental, the team whittled down their list from 14,000 to a more manageable number, each of which they systematically tested using genetic experiments in aniUsing clinical, genetic, and gene expression data as filters to distinguish genes whose copy number alteration causes cancer from those for whom copy number changes are incidental, the team whittled down their list from 14,000 to a more manageable number, each of which they systematically tested using genetic experiments in aniusing genetic experiments in animals.

For the first time, scientists have improved how a crop uses water by 25 percent without compromising yield by altering the expression of one gene that is found in all plants, as reported in Nature Communications.

The genomics era has had a profound impact on life science research, leading to significant developments such as the use of expression quantitative trait loci (eQTLs), which link polymorphisms in single genes to quantifiable changes in gene expression associated with specific diseases.

The light - activated genetic switch could be used to turn genes on and off in gene therapies; to turn off gene expression in future cancer therapies; and to help track and understand gene function in specific locations in the human body.

The researchers developed algorithms to use in a «systems biology modeling cycle,» in which they repeatedly fit a model to gene expression data obtained from laboratory experiments until a good fit was obtained between the predicted and the measured outcomes.