Winners and other images from the 2008 BioScapes Photo Competition
use light microscopes to portray extraordinary images of biological specimens
Weiner never expected to find this robust wave motion, and until recently, he would not have been able to visually capture
it using a light microscope.
Using a light microscope, researchers at the Swiss Federal Institute of Technology in Zürich watched fluorescently tagged vaccinia virus — a more benign cousin of smallpox virus — migrate toward target cells.
Not exact matches
Some six decades ago, it had been
used in the study of human chromosomes: The chemical, which could stop mitosis in its tracks, made it easier to spy the dividing chromosomal strands in metaphase, where they could be clearly viewed under a
light microscope.
His technique
uses three
microscopes in succession to magnify the sample, capture
light from the tilted plane, and sweep the
light sheet.
Because of its amplification, the resonator can improve the performance of cameras looking for tiny wisps of
light, such as those
used in
microscopes.
The
light is then measured
using a
microscope, yielding a three - dimensional map of the electric fields.
An electron
microscope, which
uses a beam of electrons as a
light source, recorded the raw data that was then analyzed to create 3 - D reconstructions of the structures.
This can be useful for everything from
using a simple
light microscope to watch cells divide to
using light - based techniques to drive genetic and cellular actions.
Using a scanning transmission electron
microscope at the Lawrence Berkeley National Laboratory's Molecular Foundry, Miao and his colleagues analyzed a small piece of tungsten, an element
used in incandescent
light bulbs.
When LCLS opened six years ago as a DOE Office of Science User Facility, it was the first
light source of its kind — a unique X-ray
microscope that
uses the brightest and fastest X-ray pulses ever made to provide unprecedented details of the atomic world.
The technology the researchers
used to tackle this problem consists of a wide - field
microscope that emits blue
light, which penetrates about 50 to 150 microns below the skin and is reflected back to a video camera.
To test this idea, Szibor's graduate student, Christoph Schubert, tracked pollen counts in his own nose for a year
using what he termed a «handkerchief test» — after blowing his nose, he would identify the types of pollen in the mucus
using light and scanning electron
microscopes.
The team monitored the passage of
light through the waveguide
using a near - field scanning optical
microscope and confirmed that a narrow beam of
light successfully passes through the waveguide forwards, but that the wave's symmetry breaks down when traveling backwards2.
Then,
using a powerful
microscope that magnifies and
lights up the electrical circuitry of the cells, they watched the chain of events that unfolded inside them.
«We can
use expansion pathology to push conventional
light microscopes beyond their current limits, which could have important applications in diagnostic pathology,» said the study's co-lead author, Octavian Bucur, MD, PhD, of the Department of Pathology and Cancer Research Institute at BIDMC, who is also a Ludwig Cancer Center Research Investigator.
The upper left electron
microscope image shows spaghetti - shaped organic polymers now
used for organic
light - emitting diodes, or OLEDs.
The cellular features
used to diagnose certain diseases are often too small to be seen through a standard
light microscope.
In addition to fruit flies, they successfully
used the program to analyze images of zebrafish and mice, as well as data collected from a commercial
light sheet
microscope and a commercial confocal
microscope.
Using this approach, it takes about an hour to scan a piece of tissue 500 by 500 by 200 microns, using a light sheet fluorescence micros
Using this approach, it takes about an hour to scan a piece of tissue 500 by 500 by 200 microns,
using a light sheet fluorescence micros
using a
light sheet fluorescence
microscope.
Using one of the world's most powerful soft X-ray
microscopes — the Scanning Transmission X-ray
Microscope (STXM) and X-ray Emission beamlines — at the Canadian
Light Source in tandem with one of the world's highest resolution aberration - corrected transmission electron
microscopes housed at the University of Illinois at Chicago (UIC), Banerjee and collaborators from the Lawrence Berkeley National Laboratory, the UIC and Argonne National Laboratory were able to observe the unique electronic properties of their novel vanadium pentoxide and directly prove magnesium - ion intercalation into the material.
The three new Nobelists overcame that limit
using fluorescence, getting the objects under the
microscope to emit
light themselves to reveal their details.
That required some special equipment: a
microscope that
uses lasers and fluorescent
lights to piece together 3D images, a special
lighting system to keep the plants healthy during their longer - than - usual time in the spotlight, and a
microscope setup flipped entirely on its side so the plants could grow upright instead of growing horizontally along a slide.
The University of Portsmouth and NREL collaborated with scientists at the Diamond
Light Source in the United Kingdom, a synchrotron that
uses intense beams of X-rays 10 billion times brighter than the sun to act as a
microscope powerful enough to see individual atoms.
Rather than the
light used in a traditional
microscope, this technique
uses focused beams of electrons to illuminate a sample and form images with atomic resolution.
«Previously, dynein movement had only been tracked by attaching fluorescent molecules to the proteins and observing the fluorescence
using very powerful
light microscopes.
But it was so small that we couldn't just
use a normal
light microscope to study it.
In contrast to the hazy images shown in some museums and television documentaries, the right
lighting and focusing can produce micrographs of startling clarity
using original
microscopes or modern replicas (see slideshow).
With much shorter wavelengths than photons of visible
light, electron beams can be
used to observe objects hundreds of times smaller than those that can be resolved with an optical
microscope.
The fluorescent
light emitted by the molecules is observed through the glass plate
using a
microscope.
Contact: 508-289-7139;
[email protected] WOODS HOLE, Mass. —
Using a simple «mirror trick» and not - so - simple computational analysis, scientists affiliated with the Marine Biological Laboratory (MBL) have considerably improved the speed, efficiency, and resolution of a
light - sheet
microscope, with broad applications for enhanced imaging of live cells and embryos.
Sections stained for markers described above were visualized
using a Leica DM6000 B upright fluorescence
light microscope with motorized Z - focusing.
The cell was then fit into a
microscope that
uses a beam of electrons, rather than
light, to obtain images.
These tools, however, produce nothing close to the angstrom - level (tenth of a nanometer) resolution of a new type of
microscope that
uses femtosecond pulses of extreme ultraviolet
light (EUV)-- the same wavelength
light to be
used for sub-10 nanometer semiconductor lithography.]
Using colorfully labeled antibodies to
light up the new protein under their
microscopes, they found that it was present where Listeria entered cells and also present at adherens junctions between epithelial cells.
Lenses are
used in
microscopes and cameras to focus
light, thus allowing a researcher to see small things or a photographer to capture image of things that are far away.
For his work on producing an acoustic
microscope using sound rather than
light in order to form images.
The sections were then stained with hemotoxylene and eosine stain and were visualized and imaged under Nikon TE - 2000S
light microscope (Nikon, Japan)
using ACT - 2U software (version 2.1)(Nikon, Japan).
And how they are doing it just might be the semiconductor industry's ticket for extending its
use of optical
microscopes to measure computer chip features that are approaching 10 nanometers, tiny fractions of the wavelength of
light.
Using a novel
microscope that combines standard through - the - lens viewing with a technique called scatterfield imaging, the NIST team accurately measured patterned features on a silicon wafer that were 30 times smaller than the wavelength of
light (450 nanometers)
used to examine them.
Toward this end we have built a mobile phone - mounted
light microscope and demonstrated its potential for clinical
use by imaging P. falciparum - infected and sickle red blood cells in brightfield and M. tuberculosis - infected sputum samples in fluorescence with LED excitation.
Using a technique called smFISH (single - molecule fluorescence in situ hybridization), the team attached dozens of small fluorescently labeled probes to each molecule of specific messenger ribonucleic acid (RNA), which causes these molecules to
light up under the
microscope.
In confocal
microscopes, optical sectioning of a specimen is based on discriminating the out - of - focus reflected
light by
using a pinhole.
Researchers
used a powerful X-ray
microscope at Berkeley Lab's Advanced
Light Source (ALS) to capture images of nerve cell samples at different stages of maturity as they became more specialized in their function — this process is known as «differentiation.»
Inverted
microscopes are
used to image lives cells in culture (flasks, dishes, or multi-well plates)
using transmitted
light, phase contrast, DIC, or fluorescence modes.
The researchers first created a bunch of mutated versions of a certain
light - sensitive protein, put them into cells, let the cells grow, and then
use an automated
microscope to take pictures of them.
Laser
Light Test Reveals Spreading Cancer Cancer imaging and breast cancer experts used advanced microscopes equipped with tissue - penetrating laser light to develop a promising new way to accurately analyze the distinctive patterns of ultra-thin collagen fibers in breast tumor tissue samples and to help tell if the cancer has sp
Light Test Reveals Spreading Cancer Cancer imaging and breast cancer experts
used advanced
microscopes equipped with tissue - penetrating laser
light to develop a promising new way to accurately analyze the distinctive patterns of ultra-thin collagen fibers in breast tumor tissue samples and to help tell if the cancer has sp
light to develop a promising new way to accurately analyze the distinctive patterns of ultra-thin collagen fibers in breast tumor tissue samples and to help tell if the cancer has spread.
Concerning SEM study a bone slice, adjacent to that
used for
light microscopy, was examined
using Cambridge 250 Mark 3 scanning electron
microscope, working at 20 kV beam voltage.
electron
microscope A
microscope with high resolution and magnification that
uses electrons rather than
light to image an object.
It includes more than 1500 images of scientific, biological, and medical subjects that were photographed
using light and electron
microscopes.