Sentences with phrase «use of amyloid»

Not exact matches

There are new types of tools that can be used to allow doctors to view the amyloid plaque while a person is still alive and see the interactions that are taking place.

To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.

Yanagisawa and colleagues are now extending and expanding their study in hopes of bringing an amyloid blood test closer to routine clinical use.

This was the first time this technology has been used on amyloid fibrils of the infectious prion, which are a special form of clumped - together proteins that form fibrils.

Several research groups have tried to clarify this issue using various methods to clear microglia from the brains of Alzheimer's mice to see how this would affect amyloid buildup.

When Gordon Lithgow at the Buck Institute for Research on Aging in Novato, California, and colleagues grew the soil - dwelling nematode Caenorhabditis elegans in agar plates soaked in thioflavin T — a dye used to visualise clusters of amyloid beta protein — they found that the worms lived 30 to 70 per cent longer than average.

The researchers suggest that their blood test serves as a cheap and simple option to pre-select individuals from the general population for further testing by these more invasive and costly methods to exclude the falsely positive subjects.The blood test developed by Gerwert and colleagues uses a technology called immuno - infrared sensor to measure distribution of pathological and healthy structures of amyloid - beta.

To better understand the presence and importance of these proteins in the urine of pregnant women with preeclampsia, the team used a dye called Congo Red, which was known to bind proteins such as amyloid based on previous research done with other protein misfolding conditions.

Using visible spectrophotometry, the scientists measured the oxidative reaction of DTBC when exposed to beta - amyloid only, beta - amyloid bound to copper, and copper - bound beta - amyloid in a mixture containing betanin.

First, the researchers used mice that had been genetically modified to produce excess amounts of the human version of ß amyloid — a common Alzheimer's disease model.

The treatment uses tiny droplets of fat, called nanoliposomes, which are coated in protein fragments that are able to stop amyloid protein accumulating into plaques, even at low concentrations.

«Using nanoliposomes offers an alternative way to inhibit the toxic build - up of amyloid plaques without activating an immune response in the brain.

Building on this work, Ming, Darrell Cole Cerrato and colleagues at the University of South Florida wanted to find out if betanin, a beet compound used in commercial dyes that readily binds to metals, could block the effects of copper on beta - amyloid and, in turn, prevent the misfolding of these peptides and the oxidation of neurons.

«We used two different antibodies — one of which has been in clinical trials for Alzheimer's — to neutralize the effects of amyloid - beta and showed that you're able to rescue changes in tau,» Young - Pearse said.

«However, we are also studying the effects of pazopanib on amyloid beta to create a better understanding of how it works and what diseases it could potentially be used to treat.»

If even trace amounts of pathogenic protein are present, they rapidly use the normal proteins to create millions of insoluble, fibrous amyloid strands.

Thus, there is an urgent need for diagnostic tools that can be used to investigate living people, and that can distinguish between deposits of amyloid beta and tau in the brain.

In the new study, Ances and colleagues used one of these tags and an amyloid - binding one to analyze deposits of both proteins in 10 people with mild AD and 36 healthy adults.

Scientists have long used an imaging technique called positron emission tomography (PET) to visualize ß - amyloid deposits marked by radioactive chemical tags in the brains of people with AD.

In a study published in the journal Cell Reports, Drs. Per Nilsson, Takaomi Saido and their team show for the first time using transgenic mice that a lack of autophagy in neurons prevents the secretion of amyloid beta and the formation of amyloid beta plaques in the brain.

To understand the possible link between beta - amyloid accumulation and sleep, the researchers used positron emission tomography (PET) to scan the brains of 20 healthy subjects, ranging in age from 22 to 72, after a night of rested sleep and after sleep deprivation (being awake for about 31 hours).

Instead of stirring up powerful T cells through an injected amyloid vaccine, they used a nasal spray containing two drugs that provoke a less robust but more manageable immune response.

In close collaboration with his TUM colleagues Johannes Buchner, professor of biotechnology and Sevil Weinkauf, professor of electron microscopy, Reif determined that the small heat shock protein uses a specific non-polar beta - sheet structure pile in its center for interactions with the beta - amyloid, allowing it to access the aggregation process in two locations at once: For one it attaches to individual dissolved beta - amyloids, preventing them from forming fibrils.

Using this experimental model, the researchers then observed the behavior of the microglia and found that the soluble form of amyloid beta stimulated microglia to engulf synapses.

Using a special imaging technique, Northwestern Medicine scientists have discovered the toxic build - up of amyloid protein is greater on the left side of the brain — the site of language processing — than on the right side in many individuals living with PPA.

«It could also be used to identify new types of amyloids and presymptomatic patients who are at risk of developing the disease,» says Hammarström and collaborator professor Peter Nilsson.

Some scientists do not like to use the word «prion» in connection with the amyloids associated with common neurodegenerative diseases, or to describe any of their properties as «prion - like» — because of its connotation of infectious, deadly disease.

The radioactive dye used, florbetapir (Amyvid), was approved by the U.S. Food and Drug Administration in 2012 for PET imaging of the brain to estimate beta - amyloid plaque density in patients being evaluated for cognitive impairment.

The study, which used both cell cultures and mouse models, analysed how quickly Amyloid beta proteins, which are thought to be a likely cause of Alzheimer's disease, entered the retina and how they damaged it.

This is a drawing representing structure of properly functioning protein (left) which is optically invisible to high power laser light, and toxic amyloid (right) responsible for brain diseases that might potentially be cured using lasers in photo therapies.

«Using this combined approach, we were able to reduce the levels of amyloid - beta, but, importantly, restored spatial learning and memory to the level observed in healthy mice,» Jankowsky said.

All previous clinical trials designed to reduce the levels of amyloid - beta using one therapy at a time have had limited success.

Using two complementary approaches to reduce the deposits of amyloid - beta in the brain rather than either approach alone improved spatial navigation and memory in a mouse model of Alzheimer's disease.

The physiologic relevance of the findings obtained using ex vivo islet studies is demonstrated in the in vivo studies by administering IAPP aggregates, i.p., in living animals and monitoring the progressive accumulation of islet amyloid and associated diabetes pathology.

Immunotherapy is a promising strategy for the treatment of Alzheimer's that uses antibodies to stimulate the immune system to remove pieces of a protein called amyloid beta which accumulates in the brain (in deposits known as plaques) and is thought to be a major factor driving Alzheimer's neurodegenerative effects.

However, despite this success, clinical trials using these antibodies caused inflammatory side effects in the brain of Alzheimer's patients, in particular amyloid - related imaging abnormalities (ARIA), which results in small bleeds and dangerous brain swelling.

S3 A), which is a widely used procedure for monitoring amyloidosis and is based on the specific interaction of ThT with amyloid structures (Levine, 1993).

For that purpose, we used aggregates of the Tau protein, implicated in Alzheimer's disease, as well as the bacterial amyloid protein microcin (Mcc).

Using microscopy, Tanzi, Moir, and their colleagues observed that amyloid - β formed fibrils and trapped C. albicans cells in the guts of infected worms.

Measuring tau deposits using the novel radiotracer 18F - AV - 1451 (18F - T807), in conjunction with 11C - PiB to measure amyloid plaques and 18F - FDG to measure regional neurodegeneration, offers new insight into the neurodegenerative characteristics of Alzheimer's disease and shows that tau pathology may be an instrumental target for disease - modifying strategies.

Visualization of fibrillar amyloid deposits in living, transgenic < i > Caenorhabditis elegans animals using the sensitive amyloid dye, X-34.

This study used the well characterized APPswe / PS1ΔE9 mouse model that exhibits a subset of behavioral and pathological features of AD, including age - dependent accumulation of beta - amyloid (Aβ) as well as learning and memory deficits [10].

Proclara has developed a pipeline of drug candidates that use GAIM to target the common amyloid protein conformation, dissociating and preventing the formation of misfolded protein assemblies, and blocking the cell - to - cell transmission of toxic aggregates.

Another limitation of this study is that the method the scientists used to detect the amyloid beta has not been thoroughly tested for detecting the types of amyloid beta present in the brains of healthy people.

Cognitive behavioral assays, histology, ELISA and Western blotting were used to assay the effect of J147 on memory, amyloid metabolism and neuroprotective pathways.

Using blood collected from elderly persons aged up to one hundred and demonstrating no cognitive impairment, the researchers isolated precisely those immune cells whose antibodies are able to identify toxic beta - amyloid plaques but not the amyloid precursor protein that is present throughout the human body and that presumably plays an important role in the growth of nerve cells.

During these years, she focused on the structural properties of the amyloid - like oligomeric intermediates formed by the bacterial HypF - N protein under different solution conditions, mainly using the Förster Resonance Energy Transfer (FRET) technique.

Identifying the molecular triggers for the onset of AD - related cognitive decline presently requires the use of suitable animal models, such as the 3xTg - AD mice, which develop both amyloid and tangle pathology.

MAIN OUTCOME MEASURES: Neuropathological and biochemical examination of the brain using standardized evaluation for tau, beta - amyloid, and synuclein deposits.

METHODS AND RESULTS: Immunohistochemistry and digital image analysis was used to examine the detailed localization of beta - amyloid (42)(A beta 42), a major component of amyloid plaques, in the entorhinal cortex and hippocampus of AD brains.