Sentences with phrase «used as a negative control»

Can be used as a negative control for other p - BABE - puro derived HOS CD4 cell lines.

An isotype control for the secondary antibody was used as a negative control in both samples.

Calpain inhibitor (1 μl) added to treated cell lysate was used as a negative control.

A sample without a primary antibody can be used as a negative control to distinguish specific and non-specific signal from the secondary antibody.

Furthermore, samples that have been genetically manipulated to alter the target's levels (e.g., CRISPR) can be used as a negative control.

Mouse ES (mES) cells were used as a negative control.

si - Control targeting EGFP sequence was used as a negative control.

A pool of normal human plasma was used as a negative control (NC).

Vero E6 cells without virus inoculation and HeLa cells without ACE2 were used as negative control.

Not exact matches

In a richly valued market, that sort of risk control is most appropriately established using call options having a strike price situated at about the point where various trend - following measures would turn negative — what is known in finance as a «contingent position» because the position creates its own exit if the market deteriorates further without an interim recovery - and particularly if it deteriorates abruptly.

There were her black - and - white negative thoughts, her rejection of help, her insistence on a definition of life as being able to take care of oneself, her use of objectified terms (such as the new life stage of «miserable existence» to replace merely «feeling miserable»), her unsolicited speaking for others, her legalistic analysis of the problem of euthanasia and doctor - assisted suicide, her exaggeration of minor and temporary discomforts, her refusal to accept family support — cumulatively resulting in her choice to be «in control» and die.

EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - used in DC - SIGN

While we could not use the XT slides with secondary antibody only as a negative control (due to the limited material), we did stain smears of ticks fed on clean mice using all antibodies (primary and secondary).

Negative controls including the secondary antibody alone and uninfected monkey tissue were used, as was an ex vivo section of infected macaque brain tissue [43] for the positive control.

Using serial dilutions of a cloned fragment of XMRV gag as a positive control, the nested PCR assay could reliably detect at least 3 copies of DNA per reaction, even when spiked into genomic DNA prepared either from 293FT cells or donor PBMCs previously validated to be negative for XMRV.

DU145 cells (XMRV - uninfected)[39], and 22Rv1 cells carrying at least 10 integrated copies of XMRV [31], were used as a negative and positive control, respectively, in XMRV FISH assays.

In all our immunohistochemical analyses of PRLR in mouse mammary tissue, we used PRLR - deficient mammary glands as a negative control to test the specificity of the staining.

Use full range of motion and perform both the positive and negative portion of the movement in a slow, controlled manner, and don't forget to squeeze the calves as hard as possible.

Learning to use negative emotions as signals and opportunities for growth and change, thus controlling them instead of letting them control you.

The main benefits of Talk a Lot are: • Students have to think in English during lessons in a controlled and focused way • Students learn how to memorise correct English structures naturally, without abstract and unrelated grammar lessons • Students learn how to construct eight different common verb forms, using positive, negative and question forms, as well as embedded grammar appropriate to their level.

As an MRO, you are prohibited from doing the following as part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances AcAs an MRO, you are prohibited from doing the following as part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances Acas part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances Act.

Practices may vary according to the type of financial information sought; the exclusion of items outside applicant's control, such as medical expenses; the time period preceding the employment application for that information; when in the selection process the information is used; and consideration of the applicant's explanation for negative information.

My study of dog bite - related fatalities occurring over the past five decades has identified the poor ownership / management practices involved in the overwhelming majority of these incidents: owners obtaining dogs, and maintaining them as resident dogs outside of regular, positive human interaction, often for negative functions (i.e. guarding / protection, fighting, intimidation / status); owners failing to humanely contain, control and maintain their dogs (chained dogs, loose roaming dogs, cases of abuse / neglect); owners failing to knowledgably supervise interaction between children and dogs; and owners failing to spay or neuter dogs not used for competition, show, or in a responsible breeding program.

The use of potassium bromide as a sole seizure control agent is no longer uncommon; however, the concurrent use of potassium bromide with phenobarbital allows for a reduction of phenobarbital use by 30 % to 50 %, which is usually enough to alleviate negative phenobarbital side effects.

While some may find it a negative that you can't control which character you are using, it does make the storytelling a lot better as the scenes can be a lot more tailored as the game progresses, and helps to give more depth to the plot.

So in a way, if adding in GHGs helps in weather prediction, one negative outcome would be that weathermen will be able to say, ``... as predicted...,» giving viewers a false sense that everything is normal & under control & GW is not happening, since I doubt they will mention that GHGs were used to help them make such good predictions.

Not all farmers are bad - players have to farm as part of the game Persistent farmers are bad - the type that do nothing but farm (since we know their gold / items are used for sale, not personal use) Not all farmers are rude The majority of farmers seem to be Chinese (see above as to why) Chinese - speaking players are only encountered when they are farming Chinese farmers are rude, train mobs, try to control an area, and are disruptive to players attempting to play in the same area Chinese farmers monopolize an area, causing other players distress Non-Chinese farmers typically do not exhibit the rudeness and other negative attitudes / behaviors

The messaging solution with built - in parental controls has arrived at a time when there's mounting concern over how use of social media has detrimental impacts on people's well - being, as well as concern over how technology companies have irresponsibly developed products aimed to addict their users without understanding the negative consequences of those actions.

Common societal misconceptions are at play here, as well as basic human psychology: parents often fear losing control of themselves or allowing their children to lose control of their negative emotions, and it is easy to fall into the trap of using distraction techniques to pacify a child who is upset.

The Dismissing Parent disengages, ridicules or curbs all negative emotions, feels uncertainty and fears feeling out of control, uses distraction techniques, feels that emotions are toxic or unhealthy, uses the passage of time as a cure - all replacement for problem solving.

A study published in Psychological Science found that when participants engaged in expressive writing (i.e., writing about their deepest emotions related to their relationship), they were more likely to use positive and negative emotion words — such as happy and love — during daily exchanges with their significant others compared to participants in a control group asked to write about simple day - to - day activities.2 Importantly, the study revealed that increases in the use of positive emotion words were associated with higher levels of relational stability.

The relationship between depressive symptoms and step count has only been assessed in specific populations with small sample sizes, such as low - socioeconomic status Latino immigrants, 16 elderly Japanese people17 or patients with chronic conditions such as heart failure18 19 or chronic obstructive pulmonary disease.20 21 Studies yield contradictory results, with some observing no association between depressive symptoms and daily step count, 19 21 while others report a negative correlation.16 — 18 20 In one cross-sectional sample of healthy older adults, an inverse association between depressive symptoms (using the Goldberg Depression Scale - 15) and accelerometer measured daily step count disappeared after controlling for general health and disability.22 While a systematic review suggests reduced levels of objectively measured PA in patients with depression, 23 it is not known whether this association is present in those at high risk of CVD and taken into account important confounding such as gender and age.

I have used time outs 2 different ways: one as a form of discipline, after which I always follow through by talking it out when he has had time to calm down, and two as a way to prompt him into trying to calm down before he «loses control» and hopefully avoiding those negative behaviors.

Finally, because negative marital quality may predict increased alcohol use rather than the reverse, we estimated an APIM in which actor negative marital quality, partner negative marital quality, gender, and all possible two - way and three - way interactions were included as predictors of average drinks per week in Wave 2, controlling for average drinks per week in Wave 1.

As a measure of maternal use of negative control a mean score across situations was calculated for the following reactions: ignoring, love withdrawal, physical punishment, and exercise of power.

Coercive control practices of authoritarian parents as well as nondemanding practices of unengaged parents and to some extent of democratic parents were related to more negative parent — child relationship indicators than was the extensive use of firm / confrontive control (rational - demanding) by directive parents or authoritative parents.