Sentences with phrase «used as antigen»

To raise antibodies against goat MSTN, the C - terminal region of goat MSTN (amino acids 266 — 375) was expressed in bacteria, purified with a nickel column and then used as antigen to immunize rabbits.

Not exact matches

Currently, rising blood levels of prostate - specific antigen (PSA), a protein made by the prostate gland, are used as indicators of tumor recurrence.

The tools included TCRdist, which researchers used to calculate the similarity and differences of key features of T cell receptors, such as amino acid sequences in important regions for antigen recognition.

The company wanted to use it as a diagnostic marker for disease: If synthetic versions of antibodies could be tagged with aequorin in the lab, then whenever they matched up with an antigen (or surface protein) of a specific pathogen in blood or tissue, the sample would glow.

A recent study by investigators from LIJ Medical Center demonstrated that using magnetic resonance imaging (MRI) in men with an elevated prostate specific antigen (PSA) resulted in a prostate cancer detection rate that was twice as high as data reported in the March 1999 Prostate journal that analyzed men undergoing the standard 12 - core biopsy with an elevated PSA.

They gauged aggressiveness using the results of the patients» prostate cancer - specific antigen, or PSA, tests, as well as the clinical stage of their cancer and Gleason grade.

Ken Shea and colleagues at the University of California, Irvine, used melittin, the toxin in bee venom, as the antigen (the substance triggering an immune reaction).

The other four candidates are tests that detect antigens to Ebola in blood and other fluids — in many cases, using the same strip format and analytical technique, called enzyme - linked immunosorbent assay (ELISA), as over-the-counter pregnancy - test kits.

Over the past two years, investigators from the Perelman School of Medicine at the University of Pennsylvania have reported results from a human trial in GBM using chimeric antigen receptor (CAR) T cell therapy, through which patients» own T cells were engineered to track down and kill cancer cells that express a tumor - specific protein known as EGFRvIII.

While the 3D injectable scaffold is being tested in mice as a potential cancer vaccine, any combination of different antigens and drugs could be loaded into the scaffold, meaning it could also be used to treat infectious diseases that may be resistant to conventional treatments.

«The retraction states that Mignot and his colleagues were unable to replicate the results of the ELISpot assay, a widely used method for measuring how immune system cells such as T cells respond to fragments of foreign proteins, called antigens,» Underwood writes.

It's hoped this latest insight will lead to the widespread use of antigen - specific immunotherapy as a treatment for many autoimmune disorders, including multiple sclerosis (MS), type 1 diabetes, Graves» disease and systemic lupus erythematosus (SLE).

The transchromosomal cows used by USAMRIID collaborator SAB Biotherapeutics, Inc. offer the opportunity to produce large quantities of antibodies that are fully «human,» meaning they will not be seen as foreign antigen by the human immune system.

Her dissertation looked at whether variants in the PSA gene — that is, the gene that codes for the PSA (prostate specific antigen) protein doctors use as a screen for the existence or recurrence of prostate cancer — had any influence on developing the cancer.

The laboratory has also all of the technologies required to produce recombinant bacteria expressing vaccine antigens to be used as non-pathogenic vaccine vectors.

Now as an Assistant Professor in Medicine at Harvard Medical School, Daniel uses membrane structure and function as a means to define B cell antigen recognition and inform vaccine design.

Moreover, UNISI produces recombinant bacteria expressing vaccine antigens to be used as non-pathogenic vaccine vectors.

The rational use of adjuvants will be studied in detail, as very often researchers use a «shotgun» approach with adjuvants, testing entire panels with their antigens in the search for improved immune responses.

Requires heat - induced antigen retrieval where aldehydes are used as fixatives.

We show that synNotch receptors can be used to sculpt custom response programs in primary T cells: they can drive a la carte cytokine secretion profiles, biased T cell differentiation, and local delivery of non-native therapeutic payloads, such as antibodies, in response to antigen.

Currently, pDNA can be used directly as a therapeutic agent (e.g., in gene therapy or generation of vaccine antigens) and indirectly for a range of applications.

[13] Highlighting this risk in the PD / AS context, a preclinical study using a nitrated AS species similar to one found in patient LB as an active vaccine antigen developed worrisome autoimmunitAS context, a preclinical study using a nitrated AS species similar to one found in patient LB as an active vaccine antigen developed worrisome autoimmunitAS species similar to one found in patient LB as an active vaccine antigen developed worrisome autoimmunitas an active vaccine antigen developed worrisome autoimmunity.

For the detection of specific antigens, the following primary antibodies were used: mouse monoclonal anti-Tuj1 (Covance, Princeton, NJ, USA) as a neuronal marker, rabbit anti-L1 (kindly provided from Dr. Asou)[52] and rat monoclonal anti-L1 (Millipore, Billerica, MA, USA) as granule cell markers, rabbit anti-calbindin D28K (Millipore) as a Purkinje cell marker, and rabbit anti-glial fibrillary acidic protein (GFAP, DAKO, Denmark) as an astrocyte marker.

His ongoing research projects include programs to develop adjuvants for use with H5N1 pandemic influenza antigens as well as West Nile Virus vaccines.

Virus particles recovered from the interface between the two sucrose layers were used as coating antigen.

Areas of focus include: understanding how tumour - reactive T cells and B cells promote patient survival in cancer; defining the effects of standard treatments on tumor immunity; and using genomic approaches to identify novel tumour mutations that can serve as target antigens for immunotherapy.

As noted above, existing cancer vaccines that use dendritic cells require extracting cells from a patient's blood, treating them with an engineered protein or nucleic acid that combines tumor antigens with immune - stimulating molecules, and returning the activated dendritic cells to the patient.

She uses antigen therapy to desensitize patients to environmental allergens such as molds, animals, pollens, and inhalants as well as chemicals and foods.

The assays, antigens and controls used are developed and validated as a component of our research.

The most commonly used vaccine against panleukopenia, herpesvirus and calicivirus is a multivalent vaccine: it contains viral antigens for several diseases together in the same dose, and is commonly abbreviated as the «FVRCP» vaccine.

The use of whole tumor RNA as the antigenic payload allows for an HLA - independent, whole antigen approach that aims to promote a polyclonal anti-tumor T cell response.