Sentences with phrase «used cell markers»

This catalog offers a detailed choice of the most commonly used cell markers and an extensive selection of antibodies used in immunology.

To verify that the individual cells they picked were pure, the researchers cultured them for several days to determine, using cell markers, whether they formed mature HSCs.

Not exact matches

Alcino Silva, distinguished professor of psychology at UCLA, has been using genetic markers and a highly miniaturized microscope to zero in on sets of brain cells in mice with such interconnected or «linked» memories.

After watching those mice thrive for several months, Lagasse repeated the experiment using fluorescent markers to trace the path of the liver cells.

The lab tracked the differentiating process using fluorescent cell imaging, which tested for the necessary markers during each step.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

In mice, this form of lipodystrophy was also characterized by «whitening» of brown fat cells, a loss of white fat, and signs of metabolic syndrome, including insulin resistance, fat tissue inflammation, dyslipidemia (elevated cholesterol and fat), increased resting energy use, and increased markers of cardiovascular disease.

Using these genes as markers, he was able to select PGCs from among other cells and study what happens to them.

Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.

Kelley and his team also used mice with fluorescent markers in different cells of the ear followed by next generation sequencing.

One common application for quantum dots is to use them as cell markers in biomedical research, says Todd Krauss, an associate professor of chemistry and optics at the University of Rochester's Institute of Optics who studies the fundamental properties of quantum dots as well as their optical properties.

«Eighty - two percent of the smooth muscle cells within advanced atherosclerotic lesions can not be identified using the typical methodology since the lesion cells down - regulate smooth muscle cell markers.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of human cardiac muscle cells.

Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.

To see if the newborn cells were indeed neurons, Gould and her colleagues used another marker called neuron - specific enolase.

Bruce Buchholz of the Lawrence Livermore National Laboratory used cellular markers for a study in which he concluded that we're more or less stuck with the number of fat cells we have at about age 20.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Researchers have discovered that a marker found on aggressive prostate cancer cells could also be used as a way to guide treatments to the cancer, according to new research presented at the National Cancer Research Institute (NCRI) Cancer Conference in Liverpool.

To investigate whether these varicosities actually contained synaptic molecules, the team expressed synaptic markers in the chandelier cells using transplantation techniques.

Scientists at The Institute of Cancer Research (ICR) used the cells of fruit flies, modified with a fluorescent marker that is activated when the cells are put under stress, to identify the signals responsible for driving up activity of the ER.

The authors believe theirs is the first study to show that mouse mammary gland tissues are sensitive to a mixture of 23 commonly used UOG chemicals, with dose - specific effects on tissue morphology, cell proliferation and induction of intraductal hyperplasias, an overgrowth of cells considered a marker for future breast cancer risk.

And by using fluorescent markers to tag these cells, they have even been able to detect them by using a simple mobile phone camera.

GFP has been utilized in numerous studies as a marker protein that can track chemical reactions within living cells, along with various other uses.

Using this marker, the green fluorescence of the nucleus increases progressively in cells in the growth phase and turns off after the division.

To trace the lineage of liver cells, scientists used a telltale marker — the cells» response to signals delivered by a known stem - cell regulator called Wnt.

This means that, in the future, these particular cells could potentially be used as a marker to monitor prostate cancer patients and predict if the disease is going to spread — alongside other monitoring techniques.

Once they were able to isolate skeletal muscle cells using the newly identified surface markers, the research team matured those cells in the lab to create dystrophin - producing muscle fibers.

Using the ERBB3 and NGFR surface markers, the skeletal muscle cells were isolated and then injected into mice at the same time a TGF Beta inhibitor was administered.

Skeletal muscle cells isolated using the ERBB3 and NGFR surface markers (right) restore human dystrophin (green) after transplantation significantly greater than previous methods (left).

Now, researchers at the University of Cambridge have used fluorescent markers to track cell development in the embryo of a cartilaginous fish — a little skate in this case — and found that these thorny scales are in fact created from the same type of cells as teeth: neural crest cells.

The group was then able to sort the cells using markers expressed on the cell surface, to help pull out successfully edited cells for research, and eventually for therapeutic use.

This biomechanical marker could be used as a rapid and cost - effective approach to enrich for stem - like cells in corneal transplant tissue.

The researchers note that CD8 + T cells could potentially be used as markers for the progression of fatty liver disease, which is expected to become the leading indication for liver transplantation within the next one or two decades.

Lin and Artandi wondered whether they could use telomerase expression as a marker to identify the subset of cells responsible for regenerating the liver during normal turnover.

It uses a specially developed fluorescent marker which attaches to cell proteins when injected into patients.

The rarity of latently infected cells and the lack of a marker for latent HIV - 1 infection necessitate the use of in vitro model systems for detailed studies of this process.

Using FACS we also found that all of the F4 / 80 + cells coexpressed the common leukocyte antigen CD45 and the monocyte lineage marker CD11b (data not shown).

Macrophages are immune cells commonly used as markers for inflammation, and their increased presence helps confirm that SNRK plays a role in regulating inflammation in white fat tissue.

In breast cancer, CSCs or tumor - initiating cells were first identified by using a combination of cell surface markers, CD24 − / CD44 + / ESA (EpCAM) + (2).

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome - DNA, epigenetic markers and RNA - during the development, maturation and disease of human cardiac muscle cells.

In this report, we indeed showed that CSCs isolated from both 231BoM and 231BrM have strong tumor - initiating abilities; however, they preferentially metastasize to bone and brain, respectively, despite the fact that these cells were isolated using the same markers (CD24 − / CD44 + / ESA +).

Pluripotent cells were cultured in mouse Noggin protein for 5 d and immunocytochemistry was used to detect retinal specific markers.

Two additional panels will be established allowing more than 60 different immune markers to be monitored at the single - cell level, followed by data analysis using SB tools available at CEA.

Using a novel methodology, the researchers found an association between the disorder and a DNA marker that is very close to the dopamine transporter gene, which controls the intake of this important neural messenger by the nerve cells that respond to dopamine's signal.

So Gaddis used mice in which regulatory T cells would be tagged with two fluorescent markers.

Retinal cell classes were identified based on their morphology, location within the retina, and using cell class specific markers (Figures 5 and S2, S3, S4, S5, S6).

Annexin V - FITC staining was used as a marker for apoptotic cells, while PI staining was used as an indicator of necrotic cells that had lost membrane integrity.

Markers were used to detect retinal cell classes.

Using Acks as a neural activity marker, we demonstrated that neural activity in the mushroom bodies, especially in Class II Kenyon cells, one subtype of mushroom body intrinsic neurons, and a restricted area between the dorsal lobes and the optic lobes was increased in the brains of Japanese honeybee workers involved in the formation of a hot defensive bee ball.

The variable marker profiles used to identify such cells must be standardized and ideally should reflect the MSC origin.