Sentences with phrase «used gene analysis»

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Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

Appearing in the journal's February 2015 issue, the study, «Phylogenetic visualization of the spread of H7 influenza A viruses» uses genomic analysis to look at the phylogenetic history of genes that assort with H7 influenza.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes.

They then used a genetic technique called quantitative trait locus analysis to search for the genes involved in generating phenolics in the most promising progeny.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

Using computational data analysis, Hughes hopes to create evolutionary trees of these genes and regulatory mechanisms in order to figure out how they work together to make cells function and how they contribute to the physiology of the organisms they are found in.

In addition, using methods for the analysis of regulatory networks developed by the Califano lab in the Center for Computational Biology and Bioinformatics, Department of Systems Biology, the researchers identified a number of transcription factors (gene regulators) that have the potential to mimic the environmental signals that trigger papillae to induce new hair growth.

In the case of CHD2, scientists collaborating with the EuroEPINOMICS RES consortium used antisense technology to rapidly generate zebrafish larvae with a partial loss of function of this gene, and were then able to detect epileptic seizures in these animals using electrographic analysis (this method is very similar to electroencephalography, or EEG, which is used to analyze seizures in humans).

Researchers from Boston University School of Medicine looked for new stroke genes using genome wide association as well as meta - analysis.

Using fungal isolates that Ali provided, Sapakhova became familiar with methods of identifying biomarkers for the tan spot toxin genes using polymerase chain reaction analysis and gel electrolUsing fungal isolates that Ali provided, Sapakhova became familiar with methods of identifying biomarkers for the tan spot toxin genes using polymerase chain reaction analysis and gel electrolusing polymerase chain reaction analysis and gel electrolysis.

These tags are detected and measured using a sensitive DNA analysis technique known as massive, parallel sequencing, providing a quantitative readout of the gene's activity levels.

Analysis of the tumor genes affected by the two drugs revealed that cabazitaxel had a greater effect on cellular division and regulation of chromatin — a spool for DNA that helps control which genes are in use and when — whereas docetaxel has a greater impact on DNA transcription and repair.

Michael Metzker, at the time a graduate student in the laboratory of Richard Gibbs at Baylor College of Medicine in Houston, compared the gene sequences of the strains to see how closely related they were, using a technique called phylogenetic analysis.

Their analysis — which used DNA data from a Neandertal woman from the Altai Mountains in Siberia (SN: 1/25/14, p. 17) and 112,338 present - day British people — confirmed some links between Neandertal heritage and human diseases made by previous studies (SN: 3/5/16, p. 18), but didn't find evidence that Neandertal gene variants contribute to obesity.

Extinct human cousins may have used some genes differently than modern people do, an analysis of Neandertal and Denisovan DNA reveals.

«In the current study, using a genome - wide analysis of DNA methylation, we identified a few PPARα target genes that underwent ligand - activated PPARα - dependent DNA demethylation during the perinatal period and whose DNA methylation status persists into adulthood,» explains a corresponding author Koshi Hashimoto.

Using advanced computer analysis techniques designed to study and process biological data, the researchers identified SND1 among a handful of other genes that miR - 184 helps regulate.

Using a genome - wide association study (GWAS) that includes 1600 individuals living in Tanzania, Botswana, or Ethiopia, the authors identified regions of the genome that contribute to skin color variation and carried out a series of analyses to pinpoint the responsible genes.

Using the Single - Cell Automatic Analysis and Isolation System developed in 2013, this group isolated single OSNs responding to specific odorants in a time - lapse single - cell - array cytometric manner and identified the OR gene through single cell polymerase chain reaction (PCR).

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

Next, iCAGES cross-references these variants to databases of known cancer - causing genes, using statistical analyses and machine learning techniques to prioritize the most likely driver genes.

The researchers used tools of epigenomic analysis to trace the specific epigenetic switches controlling each of thousands of genes in both mouse and human retinal cells as the cells progressed through development.

Introns outperform exons in analyses of basal avian phylogeny using clathrin heavy chain genes

This is crucial for phylogenomic inference, as many studies [including transcriptome analyses (19, 74)-RSB- use only protein - coding genes to infer species trees.

Indeed, the team identified high variation in adjacent «normal» tissue samples, which are typically used as control samples for comparison in analyses based on mean gene expression.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Erlich used a multitude of statistical genetic and integrative genomics analyses to reveal that STRs have a function: they act like springs or knobs that can expand and contract, and fine - tune the nearby gene expression.

They then used genome - wide analysis to search for genes switched on by KLF4 at a late stage of reprogramming.

Using single cell RNA sequencing analysis, the Cairns lab profiled cells individually, establishing the gene expression profile in human sperm stem cells.

Using biopsies of the patients» tumors collected before the start of treatment and at the time patients developed resistance, the researchers performed large - scale genomic analyses to search for mutations specific to the cancer cells in all of each patient's 20,000 genes.

«Using the genome data analysis methods developed by co-author Steve Horvath at UCLA, we have uncovered crucial gene networks and we can now predict possible future genetic disorders at the eight - cell stage.»

Using the CAGE (Cap Analysis of Gene Expression) high - throughput method, the scientists determined the starting points of transcription of many thousands of genes in various phases of embryonic development of zebrafish.

In their analysis, the Heidelberg group, together with a group from the University of British Columbia in Canada, showed that the cnidaria and dinoflagellates use entirely different genes and proteins to construct their respective miniature harpoons.

The researchers collected liver samples, and looked at genes in the liver affected by e-cigarettes using a technique called RNA sequence analysis.

In previous research, Grice and colleagues illuminated the great diversity of bacteria living on the skin using DNA - based sequence analysis of bacterial genes.

A: Sheltzer: Professionally, we're working on a paper together using Joan's data - analysis ability to parse through gene - expression data from more than 20,000 cancer patients.

His group uses genome - wide and gene - specific DNA methylation analysis to identify aberrantly silenced tumor suppressor genes in B cell leukemias and lymphomas.

Real - time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven use in gene expression analysis at both low and medium throughput.

Previous genetic studies have examined the association of aspirin, NSAIDs, or both with colorectal cancer according to a limited number of candidate genes or pathways.6 - 10 Thus, to comprehensively identify common genetic markers that characterize individuals who may obtain differential benefit from aspirin and NSAIDs, we conducted a discovery - based, genome - wide analysis of gene × environment interactions between regular use of aspirin, NSAIDs, or both and single - nucleotide polymorphisms (SNPs) in relation to risk of colorectal cancer.

Further, we introduced computational methods using information theoretic measures and statistical longitudinal analysis techniques that allowed us to identify and localize significant selection of donor genes over time.

Using this «Ribotag» approach tissue - specific changes under DR have been measured in muscle, fat, gut, heart, neurons, germline and malpighian tubules, showing a highly tissue specific response upon DR.. We are developing mechanistic models of gene expression regulation and conducting comprehensive bioinformatics analyses on UTRs, promoters and the coding sequences of various elements of differentially regulated genes.

Variable genes were determined using in - house analysis software compared with the Immunogentics V gene dataset and the IMGT search engine (Ehrenmann et al., 2010; Lefranc et al., 2009).

Scientists for years have looked for the biological roots of the problem using tools such as genome - wide association studies and gene - linkage analysis, which crunch genetic and health data from thousands of people in an effort to pinpoint disease - causing genetic variants.

Moreover, our previous study found that regular use of aspirin after diagnosis was associated with longer survival among the 15 % to 30 % of patients with colorectal cancer and with a mutation in phosphatidylinositol -4,5-bisphosphate 3 - kinase, catalytic subunit alpha (PIK3CA [NCBI Entrez Gene 5290]-RRB-, one of the PI3K family genes.30 Markedly improved survival associated with aspirin according to PIK3CA status was also found in an analysis within a separate clinical trial cohort.31 Further investigations for the joint effect of these genes would be helpful to better understand the underlying molecular mechanisms of aspirin, NSAIDs, and colorectal cancer.

In the current study, the researchers used infradian cyclic locomotor activity in the mutant mice as a proxy for mood - associated changes, and examined their molecular basis in the brain by conducting prediction analyses of the gene expression data.

Detailed phylogenetic analyses of several virus genes, some of which used complete CDV genomes, revealed that strains from lions and hyenas during the 1993/1994 epidemic were strongly distinct from those in domestic dogs and wild canids.

For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).

Using this analysis, the researchers identified deletions in the coding region of the gene (in exons E2 to E9, E5 to E9, E8, E10, E14, and E1 to E15), duplications (E11 to E12) on two chromosomes, and a nonsense mutation.

Scientists who are trained in the Carlezon lab develop expertise in the analysis of behavior in rats and mice, the use of genetic engineering to control or modify gene and protein expression, and molecular techniques to measure molecular adaptations.