Sentences with phrase «used gene engineering»

When the researchers used gene engineering techniques to knock out DDX3 expression in laboratory - grown cell cultures that highly expressed this protein, cell proliferation was half that of cell cultures with high DDX3 expression.

Can science use the genes engineered by nature instead of genetic engineering in the face of a pending food crisis?

Not exact matches

Synthego was founded by two former SpaceX engineers who are working to make gene editing faster and easier to use.

Scientists could search within them for resistance genes and other desirable traits to use in engineering and breeding programs.

Then they can use classical plant breeding or genetic engineering to transfer those genes into desired cultivars.

«[Some] projects aim to edit the genome of citrus trees using CRISPR — Cas9 to make them more resistant to the pest, or engineer trees to express defense genes or short RNA molecules that prevent disease transmission.

As a rule of thumb, plant breeders in both public research institutes and private companies use genetic engineering only in the following situations: 1) the gene of interest is not found within the crop gene pool, 2) if found in the gene pool, transfer will be difficult due to incompatibility and other breeding problems or 3) the transfer of the gene will take a long time as in the case of perennial crops like coconut.

The newer process of genetic engineering, which involves inserting genes from unrelated species into a plant's genome to add desirable traits, has been used in crops such as corn, soy, and potatoes.

Mead Johnson uses the term «non-GMO» to mean that the ingredients that have come from sources that have not been genetically engineered or created through biotechnology / gene splicing.

The aim of current de-extinction efforts is to use gene - editing tools to engineer living species to re-create extinct cousins, such as engineering a woolly mammoth from an elephant.

Researchers led by Xiao - Jiang Li, MD, PhD and Shihua Li, MD, at Emory University School of Medicine, used genetically engineered mice in which the huntingtin gene can be deleted, triggered only when the mice are given the drug tamoxifen.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Using CRISPR, a tool for precise genetic engineering, they modified the GLP1 gene.

Cobbling together the genes of three different species, chemical engineer Jay Keasling (Discover's 2006 Scientist of the Year) of the University of California at Berkeley transformed a metabolic pathway in yeast that allows the engineered microbe to produce a precursor to artemisinin, a compound used to treat malaria.

The virus, redesigned using sophisticated protein engineering techniques, works: With its shield and its adapter, these viral gene shuttles efficiently infected tumor cells in laboratory animals.

The idea is that activists in the rich countries will organise consumer boycotts of genetically engineered foods, while the farmers in the South oppose the patenting of genes for use in agriculture.

Ecologist Ron Thresher got a sense of how the public might react to gene drives when he described his plan to use genetic engineering to rid the Australian waterways of invasive European carp, a voracious fish that can turn a crystal - clear stream into «a disgusting mudhole.»

Immune cells called CAR - T cells have already been engineered using other gene - editing technologies.

To trace individual neurons, Jeff Lichtman of Harvard uses engineered mice whose DNA includes jellyfish genes so that the cells contain fluorescent proteins.

For example, genetically engineered organisms used in the research laboratory to develop new chemical synthesis methods are not likely to require the same level of public dialogue as products that have more uncertainty associated with them, such as organisms with gene drives, which enhance organisms» ability to pass certain genetic traits on to their offspring.

In addition, Kuchina and colleagues used biotechnology to engineer mutant forms of B. subtilis in which the two critical genes were located near one another.

In this study, published in the October 31 issue of the Proceedings of the National Academy of Sciences, Sudhir Yadav PhD, a neuroimmunology post-doctoral fellow in the laboratories of Drs. Kouichi Ito, associate professor of neurology, and Suhayl Dhib - Jalbut, professor and chair of neurology, tested mice that were engineered to have a pre-disposition for MS. Because mice would not normally develop MS, researchers used MS - associated risk genes from real patients to genetically engineer mice for this study.

His team is trying to engineer the plant Arabidopsis (a weed often used for genetic experiments) to use CAM by inserting the relevant genes into the plant.

The live - attenuated vaccine, VSV - EBOV, uses genetically engineered vesicular stomatitis virus (VSV) to carry an EBOV gene that has safely induced protective immunity in macaques.

It did not begin to seriously discuss the risks associated with using the approach to engineer genes that could quickly spread through wild populations — known as gene drives — until after experiments demonstrating the concept in fruit flies had been published in a peer - reviewed journal (V. M. Gantz & E. Bier Science 348,442 — 444; 2015).

To boost the staying power of the engineered T cells, the UPenn group wants to use CRISPR to disrupt the gene for a protein called PD - 1.

Now, a team led by microbiologist Tim Kunkel of The Rockefeller University in New York City has shown that at least two crops — lettuce and tobacco — can be engineered without using antibiotic resistance genes.

The proposed clinical trial, in which researchers would use CRISPR to engineer immune cells to fight cancer, won approval from the Recombinant DNA Advisory Committee (RAC) at the U.S. National Institutes of Health, a panel that has traditionally vetted the safety and ethics of gene therapy trials funded by the U.S. government and others.

But rather than delivering the entire gene for the clotting - factor proteins to cells, as most gene therapies do, the researchers used the viruses to engineer immune - regulating B cells to express a fragment of the clotting factor fused to an immune molecule called an immunoglobulin.

In their animal experiments, the team used genetic engineering techniques to knock out the DAXX gene, but they want to develop a drug that can achieve the same result.

Using cell models and genetically engineered mice, the authors then could reproduce kidney disease changes upon expression of APOL1 gene variants, but the disease required the presence suPAR.

They used the gene editing technology CRISPR to engineer a series of human embryonic stem cell lines, which were identical apart from the number of DNA repeats that occurred at the ends of their HTT genes.

Using a specialized gene manipulation technique called chromosome engineering, researchers developed genetically engineered mouse strains in three separate iterations: MYC only, the rest of the region containing PVT1 but without MYC and the pairing of MYC with the regional genes.

Michael Wheeler, left, and Ali Deniz Güler engineered a synthetic gene that, used in conjunction with a magnetic field, allows them to control neural circuits.

That could be good news for researchers hoping to use engineered «gene drives» to eliminate mosquito - borne diseases and invasive species.

The idea of using engineered viruses to deliver healthy genes has fuelled experiments since the 1990s.

Mice normally resist the Zika virus, so the team used animals engineered with a crippled version of an immune gene that naturally would protect them from Zika.

Analogous to integrated circuits that underlie myriad electronic products, engineered gene circuits can be used to generate defined dynamics, rewire endogenous networks, sense environmental stimuli, and produce valuable biomolecules.

Having isolated the gene, says Gilliham, they can now try putting it in other crop plants as well, using genetic engineering.

The team crossed Einkorn with modern durum wheat — the kind used to make pasta — to introduce the pump gene without genetic engineering.

Yet they would be nearly impossible to create without genetic engineering used to evaluate gene function, said Nora Lapitan, a wheat geneticist at Colorado State University.

He studied plasmids, tiny ringlets of DNA that genetic engineers use to insert foreign genes into bacteria.

The researchers used CRISPR gene editing technology to engineer stem cells that produced fluorescently labeled inner ear sensory cells.

«We may have discovered a major step toward developing a «dream tool» for remotely controlling neural circuits, by manipulating specific cells using engineered gene products that respond to magnets,» said Ali Deniz Güler, a UVA biology professor who led the study in his neuroscience lab.

The work involves using an engineered chunk of DNA instead of a virus to introduce factors into a cell that will turn on genes needed for pluripotency.

The remarkable turnaround in gene therapy is largely due to scientists» increasingly refined ability to engineer the viruses used to deliver healthy genes to the cells that need them.

This system could be used in combination with hundreds of existing mouse strains that have been engineered to express known cancer genes, allowing researchers to study more thoroughly the interactions of multiple genes.

Aiming ultimately to make healthier beef, eggs, and other farm products, scientists have used a worm gene to genetically engineer mice whose tissues are unusually rich in the heart - healthy fats found mainly in fish.

They plan to use these findings to inform research focused on controlling and manipulating gene activity in genetically - engineered bacteria, which have many important medical and industrial applications.

Scientists who are trained in the Carlezon lab develop expertise in the analysis of behavior in rats and mice, the use of genetic engineering to control or modify gene and protein expression, and molecular techniques to measure molecular adaptations.