Sentences with phrase «used gene expression analyses»

They hypothesized that mind - body interventions reverse the expression of genes involved in inflammatory reactions caused by stress, and so they conducted a systematic review of 18 different studies that used gene expression analysis in mind - body interventions.

Not exact matches

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

Indeed, the team identified high variation in adjacent «normal» tissue samples, which are typically used as control samples for comparison in analyses based on mean gene expression.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Erlich used a multitude of statistical genetic and integrative genomics analyses to reveal that STRs have a function: they act like springs or knobs that can expand and contract, and fine - tune the nearby gene expression.

Using single cell RNA sequencing analysis, the Cairns lab profiled cells individually, establishing the gene expression profile in human sperm stem cells.

Using the CAGE (Cap Analysis of Gene Expression) high - throughput method, the scientists determined the starting points of transcription of many thousands of genes in various phases of embryonic development of zebrafish.

A: Sheltzer: Professionally, we're working on a paper together using Joan's data - analysis ability to parse through gene - expression data from more than 20,000 cancer patients.

Real - time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven use in gene expression analysis at both low and medium throughput.

Using this «Ribotag» approach tissue - specific changes under DR have been measured in muscle, fat, gut, heart, neurons, germline and malpighian tubules, showing a highly tissue specific response upon DR.. We are developing mechanistic models of gene expression regulation and conducting comprehensive bioinformatics analyses on UTRs, promoters and the coding sequences of various elements of differentially regulated genes.

In the current study, the researchers used infradian cyclic locomotor activity in the mutant mice as a proxy for mood - associated changes, and examined their molecular basis in the brain by conducting prediction analyses of the gene expression data.

For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).

Scientists who are trained in the Carlezon lab develop expertise in the analysis of behavior in rats and mice, the use of genetic engineering to control or modify gene and protein expression, and molecular techniques to measure molecular adaptations.

Gene Set Enrichment Analysis (GSEA) is a microarray data - mining technique used to determine whether there is coordinated differential expression or «enrichment» in a set of functionally related genes when comparing control and experimental samples [35].

He group is also interested in high throughput gene expression data analysis, especially using Bayesian network (BN) approaches.

This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA microarray analysis.

Using gene expression profile (GEP) tests like Oncotype DX to inform treatment decision - making for breast cancer is likely to be less cost - effective in community oncology practice than previous estimates had suggested, according to an economic simulation analysis published in the Journal of Clinical Oncology.

Affymetrix GeneChips are probably the most widely used platform for gene expression analysis.

We have developed new BN algorithms and tools for analysis of gene interaction networks using high throughput gene expression data.

We applied gene set enrichment analysis (GSEA)(23) to expression array data using KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways.

The final set of covariates used for regression analysis of PMI and gene expression is presented in Supplementary Table 5.

These results suggest that the effect of PMI on measured gene expression is relatively modest and can be further minimized by using appropriate covariate correction in analyses.

Dr Gilchrist said: «This study suggests that we may improve significantly on the widely used analysis methods for determining gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in gene expression level, a measure widely used to determine the consequence of genetic, chemical or physical disturbances in living systems.»

In small cell lung cancer, starting from bioinformatics analyses of large gene expression datasets, we clustered subsets of co-expressed gene modules, derived networks of transcription factors and simulated their dynamics using logic - based mathematical modeling.

The BioMark Dynamic Array microfluidic qPCR system (Fluidigm Corporation, San Francisco, CA) was used for gene expressions analysis of oocytes and cumulus cells.

Using comparative genomics analysis, characterization of conserved domains and active sites, and expression analysis, 42 key genes involved in FA biosynthesis in oil palm were identified.

These webtools make use of the multilayered datasets from the BXD mouse population to expedite in silico gene function prediction through a series of integrative and complimentary systems analytical approaches, including (expression - based) phenome - wide association, transcriptome - / proteome - wide association, and (reverse --RRB- mediation analysis.

The webtools make use of the multilayered datasets from the BXD mouse population to expedite in silico gene function prediction through a series of integrative and complimentary systems analytical approaches, including (expression - based) phenome - wide association, transcriptome - / proteome - wide association, and (reverse --RRB- mediation analysis (Figure 1 - 2).

Finally, the researchers used microarray analysis to investigate the gene expression and molecular pathways in the muscles of DS mice.

The summary below highlights the applications of commonly used custo... Keywords: Extinction coefficient, Gene expression, High performance liquid chromatography, Microarray Analysis, Polymerase chain reaction - quantitative

The expression of germ cell marker genes in monkey ES cells and tissues was examined using an RT - PCR analysis.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

Since there is a higher than 95 % chance that cluster assignments are accurate (Supplemental File S2), and our validation analysis shows that 90.7 % of the array expression patterns match the RNA analysis results using other techniques (e.g., Q - PCR), we estimate that more than 86 % of the genes in a cluster follow the corresponding average expression profile.

For the analysis of gene expression in blastocysts, total RNA was isolated and purified from three pools of 10 blastocysts produced in vivo or in vitro (KSOM supplemented with 10 % FCS or in the presence of 1 g / L BSA) using the RNeasy Micro Kit (Qiagen)[18].

We combine biochemical, structural, cellular and functional information using purified proteins, mutant and transgenic plants, yeast and chemical genomic screening systems, transient gene expression assays, confocal microscopy and in silico data analysis to compare ROP - centered kinase signaling during cell polarity (in vitro pollen tubes), morphogenesis (whole plant) and pathogenesis (fungi - infected cells).

Provided data analysis that included statistics package development with staff statisticians, and high throughput assay advent and development (immediate early gene expression); Taq - man primer - probe research and design using primer express.