They hypothesized that mind - body interventions reverse the expression of genes involved in inflammatory reactions caused by stress, and so they conducted a systematic review of 18 different studies that
used gene expression analysis in mind - body interventions.
Not exact matches
Other labs have
used DNA microarray
analysis to characterize
gene expression changes in this model.
The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be
used in any type of single - cell
gene -
expression analysis.
Indeed, the team identified high variation in adjacent «normal» tissue samples, which are typically
used as control samples for comparison in
analyses based on mean
gene expression.
In the lab, the scientific team
used an approach that combined functional RNAi
analysis with
gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.
Erlich
used a multitude of statistical genetic and integrative genomics
analyses to reveal that STRs have a function: they act like springs or knobs that can expand and contract, and fine - tune the nearby
gene expression.
Using single cell RNA sequencing
analysis, the Cairns lab profiled cells individually, establishing the
gene expression profile in human sperm stem cells.
Using the CAGE (Cap
Analysis of
Gene Expression) high - throughput method, the scientists determined the starting points of transcription of many thousands of
genes in various phases of embryonic development of zebrafish.
A: Sheltzer: Professionally, we're working on a paper together
using Joan's data -
analysis ability to parse through
gene -
expression data from more than 20,000 cancer patients.
Real - time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven
use in
gene expression analysis at both low and medium throughput.
Using this «Ribotag» approach tissue - specific changes under DR have been measured in muscle, fat, gut, heart, neurons, germline and malpighian tubules, showing a highly tissue specific response upon DR.. We are developing mechanistic models of
gene expression regulation and conducting comprehensive bioinformatics
analyses on UTRs, promoters and the coding sequences of various elements of differentially regulated
genes.
In the current study, the researchers
used infradian cyclic locomotor activity in the mutant mice as a proxy for mood - associated changes, and examined their molecular basis in the brain by conducting prediction
analyses of the
gene expression data.
For
expression analysis in mice, we
used microarray data as described above to select two internal control
genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).
Scientists who are trained in the Carlezon lab develop expertise in the
analysis of behavior in rats and mice, the
use of genetic engineering to control or modify
gene and protein
expression, and molecular techniques to measure molecular adaptations.
Gene Set Enrichment
Analysis (GSEA) is a microarray data - mining technique
used to determine whether there is coordinated differential
expression or «enrichment» in a set of functionally related
genes when comparing control and experimental samples [35].
He group is also interested in high throughput
gene expression data
analysis, especially
using Bayesian network (BN) approaches.
This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in
gene expression profiles, identified
using a DNA microarray
analysis.
Using gene expression profile (GEP) tests like Oncotype DX to inform treatment decision - making for breast cancer is likely to be less cost - effective in community oncology practice than previous estimates had suggested, according to an economic simulation
analysis published in the Journal of Clinical Oncology.
Affymetrix GeneChips are probably the most widely
used platform for
gene expression analysis.
We have developed new BN algorithms and tools for
analysis of
gene interaction networks
using high throughput
gene expression data.
We applied
gene set enrichment
analysis (GSEA)(23) to
expression array data
using KEGG (Kyoto Encyclopedia of
Genes and Genomes) pathways.
The final set of covariates
used for regression
analysis of PMI and
gene expression is presented in Supplementary Table 5.
These results suggest that the effect of PMI on measured
gene expression is relatively modest and can be further minimized by
using appropriate covariate correction in
analyses.
Dr Gilchrist said: «This study suggests that we may improve significantly on the widely
used analysis methods for determining
gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in
gene expression level, a measure widely
used to determine the consequence of genetic, chemical or physical disturbances in living systems.»
In small cell lung cancer, starting from bioinformatics
analyses of large
gene expression datasets, we clustered subsets of co-expressed
gene modules, derived networks of transcription factors and simulated their dynamics
using logic - based mathematical modeling.
The BioMark Dynamic Array microfluidic qPCR system (Fluidigm Corporation, San Francisco, CA) was
used for
gene expressions analysis of oocytes and cumulus cells.
Using comparative genomics
analysis, characterization of conserved domains and active sites, and
expression analysis, 42 key
genes involved in FA biosynthesis in oil palm were identified.
These webtools make
use of the multilayered datasets from the BXD mouse population to expedite in silico
gene function prediction through a series of integrative and complimentary systems analytical approaches, including (
expression - based) phenome - wide association, transcriptome - / proteome - wide association, and (reverse --RRB- mediation
analysis.
The webtools make
use of the multilayered datasets from the BXD mouse population to expedite in silico
gene function prediction through a series of integrative and complimentary systems analytical approaches, including (
expression - based) phenome - wide association, transcriptome - / proteome - wide association, and (reverse --RRB- mediation
analysis (Figure 1 - 2).
Finally, the researchers
used microarray
analysis to investigate the
gene expression and molecular pathways in the muscles of DS mice.
The summary below highlights the applications of commonly
used custo... Keywords: Extinction coefficient,
Gene expression, High performance liquid chromatography, Microarray
Analysis, Polymerase chain reaction - quantitative
The
expression of germ cell marker
genes in monkey ES cells and tissues was examined
using an RT - PCR
analysis.
(A) The
expression patterns of germ cell marker
genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined
using an RT - PCR
analysis.
Since there is a higher than 95 % chance that cluster assignments are accurate (Supplemental File S2), and our validation
analysis shows that 90.7 % of the array
expression patterns match the RNA
analysis results
using other techniques (e.g., Q - PCR), we estimate that more than 86 % of the
genes in a cluster follow the corresponding average
expression profile.
For the
analysis of
gene expression in blastocysts, total RNA was isolated and purified from three pools of 10 blastocysts produced in vivo or in vitro (KSOM supplemented with 10 % FCS or in the presence of 1 g / L BSA)
using the RNeasy Micro Kit (Qiagen)[18].
We combine biochemical, structural, cellular and functional information
using purified proteins, mutant and transgenic plants, yeast and chemical genomic screening systems, transient
gene expression assays, confocal microscopy and in silico data
analysis to compare ROP - centered kinase signaling during cell polarity (in vitro pollen tubes), morphogenesis (whole plant) and pathogenesis (fungi - infected cells).
Provided data
analysis that included statistics package development with staff statisticians, and high throughput assay advent and development (immediate early
gene expression); Taq - man primer - probe research and design
using primer express.