Sentences with phrase «used gene sequencing»

Using gene sequencing tools, scientists from Johns Hopkins Medicine and the University of British Columbia have found a set of genetic mutations in samples from 24 women with benign endometriosis, a painful disorder marked by the growth of uterine tissue outside of the womb.

More than two decades ago, in one of the first papers using gene sequences to find signatures of natural selection, scientists hypothesized that a molecular change in an enzyme gave the Drosophila melanogaster fruit fly species its superior ability to metabolize alcohol.

Scientists use gene sequences to screen for gene's roles in diseases and important biological processes.

The researchers also hope to use gene sequencing technology to compare the brains of those who have died of Alzheimer's with those of people who died without neurological diseases, in the hope of finding DNA sequences of particular microbes in the diseased brains.

Using gene sequencing, the researchers learned that every member of the family with congenital heart disease had the same mutation in GATA4 — a change in a single letter in the gene.

«Now that we have the gene, we can actually use that gene sequence to temporarily silence the gene and make rye and other chromosomes pair with wheat and transfer genes by a natural method into wheat without calling it GMO,» Gill said.

We also examined whether rare loss - of - function mutations in NOS3 and GUCY1A3 were associated with coronary heart disease using gene sequencing data from the Myocardial Infarction Genetics Consortium (n = 27,815).

Not exact matches

That's not to say gene editing is new (it isn't), but Crispr simplifies the process by using molecular scissors that can be precisely targeted to snip out aberrant regions of genetic code, which can then be replaced with the correct sequences.

It is a term used by people grasping at straws to continue dismissing evolution even as the evidence builds faster than ever at the gene sequencing level (ie, most of you agree that drug resistant bacteria have evolved but the term MACRO evolution is misused intentionally so you could continue to dismiss science to save faith; pun intended).

Using gene - sequencing technology, researchers established that the precise segment of the pup's DNA «switched on» by the act of grooming was the part that controlled the future function of the hippocampus, which processes stress hormones.

Stool microbiome composition was characterized using next - generation sequencing of the 16S rRNA gene.

Main Outcomes and Measures Stool microbiome composition was characterized using next - generation sequencing of the 16S rRNA gene.

The reliability and stability of these methods were described by Wu et al. 27 Illumina tag sequencing of the 16S rRNA gene V4 - V5 hypervariable region was performed at the Marine Biological Laboratory in Woods Hole, Massachusetts, using established methods.28, 29 Details of sequencing methods, quality control and filtering, and statistical modeling are presented in the eAppendix in the Supplement.

The CRISPR approach is very good at disabling genes, but using the technique to fix them is much harder, because it involves replacing a faulty sequence with another.

Using a gene - sequencing technique known as whole - exome sequencing that targets only the DNA that encodes proteins, the researchers cataloged virtually every significant DNA variant in three families in which the condition is common.

In addition, they also used exome sequencing and RNAseq — sequencing directly targeted to the SHIP1 gene — to examine the structure of the SHIP1 gene at both the DNA and the RNA level.

The vaccine can be targeted to the chosen pathogen by using RNA molecules which share sequence identity with the pest's genes and prevents their expression.

Using Atlantic killifish (Fundulus heteroclitus), the researchers examined whether mitochondrial - nuclear interactions alter the frequency of alternative forms of a gene that arise by mutation, called alleles, for over 11,000 nuclear DNA sequence variations within a population of the fish with mixed ancestry.

«If we could use gene editing to remove the sequences in an embryo that cause sickle cell disease or cystic fibrosis, I would say not only that we may do so, but in the case of such severe diseases, we have a moral obligation to do so.»

In the new research, the UB scientists found they could reverse those social deficits with a very low dose of romidepsin, which, they found, restores gene expression and function using an epigenetic mechanism, where gene changes are caused by influences other than DNA sequences.

Then, using the CRISPR - Cas gene - editing tool, they spliced sequences corresponding to individual video pixels into the genome.

I had zero computer experience; in fact, I could barely type, but a postdoc in the lab helped me do a BLAST search using my gene's protein sequence as a query.

These are sequences made in the lab from RNA — the template used to produce the proteins that genes code for.

Since messenger RNA is nothing but the imprint of the gene whose message it is carrying, it is possible to use it to chemically generate the DNA sequence or gene it corresponds to.

Brains of individuals who died with Huntington's, Parkinson's or no neurological condition were analyzed using sequencing technology that provides a data readout of the activity of all genes in the genome.

Use or production of a full - length gene in which the listed sequence constitutes a short embedded part would fall within the purview of a claimed patent...

The policy — in the form of first Office Actions on a series of applications for patents on expressed sequence tags, ESTs — could greatly complicate basic gene therapy research by substantially allowing patents for small sequences of a gene that may later be used by the patent holder to corner ownership and uses of entire genes.

Genes in Space - 1 marked the first time the PCR was used in space to amplify DNA with the miniPCR thermal cycler, followed shortly after by Biomolecule Sequencer, which used the MinION device to sequence DNA.

Given this interpretation, use or production of a full - length gene in which the listed sequence constitutes a short embedded part falls within the purview of the claim.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

Using RNA sequencing, the researchers found multiple genes whose abnormal expression could lead to the high rate of death for cloned embryos, including failure to implant in the uterus and failure to develop a normal placenta.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

Sequencing the genome of one such organism, King and her colleagues found genes that code for pieces of the same proteins used for the binding of cells and communication between cells in animals — functions that would be unexpected in such an organism.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes.

Theoretically, it could be simpler and more targeted to use gene blueprints from sequencing data to build something that mimics microRNAs — binding to the same things the microRNA would — then embarking on a fecal transplant and ensuring it is safe.

The pilot project tested a dozen or so of the most commonly used gene promoters (regions of DNA that facilitate gene transcription) and segments of DNA that encode ribosome - binding sites (sequences of messenger RNA that control protein translation) to determine whether they behave consistently in different cellular contexts.

Using high - throughput DNA sequencing, the researchers located mutations in bacterial genes that resisted the toxicity of growth inhibitors produced by bacterial viruses.

The international team used a common computer system so that researchers from around the world could analyze the gene sequences based on their areas of expertise.

Using mass DNA sequencing, he recently identified resistance genes even in bacteria in pristine permafrost and deep caves.

The researchers then compared the expression of all genes in six parts of the brain of the two bird species using state - of - the - art molecular techniques, including next - generation sequencing — the first time these tools have been used to find brain properties related to innovation and problem - solving in wild birds.

Using a novel combination of technologies, including trio exome sequencing of patient / parental DNA and genetic studies in the tiny larvae of zebrafish, the EuroEPINOMICS RES consortium found that mutations in the gene CHD2 are responsible for a subset of epilepsy patients with symptoms similar to Dravet syndrome — a severe form of childhood epilepsy that is in many patients resistant to currently available anti-epileptic drugs.

So to reveal their function, biologists used the sequence information along with a technique called RNA - mediated interference (RNAi) to temporarily inactivate each of nearly 17,000 of the worm's genes.

The goal of the human genome project was to use DNA sequencing to reveal all three billion DNA letters in our chromosomes and find all our genes.

In this study that used deep DNA sequencing of bacterial specific genes, the guts of both males and females exposed to lead had all of the similar complexity in microbiota as those not exposed.

Gai and Falk used the high - performance computer cluster at Loyola's Center for Biomedical Informatics to analyze billions of DNA sequences to identify the gene mutation in the child and her parents.

Medical researchers have used DNA sequencing to identify a gene variant responsible for causing lupus in a young patient.

With the state - of - the - art DNA sequencing technology used in this study, they will be able to pinpoint the exact mistake in each patient's SS - related genes.

To confirm and strengthen the findings, Zwick and his team are currently performing an independent study of individuals with Down syndrome, using whole genome sequencing to further delineate alterations in genes that perturb heart development in children.