Sentences with phrase «used human cell cultures»

To study that protein's role, they used human cell cultures.

Not exact matches

While other papers have examined these mutations using expensive and time - consuming experiments on live ferrets and laboratory cell cultures, Deem and Melia Bonomo used the pEpitope method to rapidly calculate how much the egg - passage mutations would decrease vaccine efficacy in humans.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Using human fetal «mini-brains» grown in 3 - D cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).

The team used human embryonic stem cells — which can transform into any cell of the body — and cultured them in a mixture of chemicals to grow human brain cells.

Human chondrosarcoma HCS - 2 / 8 cells, which are a type of benign bone cancer cells, can be used to investigate how optimization of culture conditions could improve the synthesis of cartilage - specific molecules.»

Cultured human cells are a better alternative and are already used by one manufacturer, says the report.

Studies of IBD are typically performed using cell culture experiments or animal models, which don't mimic the precise conditions that occur in the gut of human patients.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

Although electrolyte leakage is still undesired, its danger is minimized by the use of either the normal saline solution pumped into the body in most IV treatments or a cell - culture medium that contains amino acids, sugars, and vitamins in addition to sodium ions and thus mimics the fluid that surrounds human cells.

To replicate these cell culture results, Rani used human stem cells to grow neurons into what is called a mini brain.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

In a paper published in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated cell cultures to sustain hepatitis B virus in human liver cells, allowing them to study immune responses and drug treatments.

Cell culture systems used today also do not faithfully represent the features of human neurons.»

Using human T cells in culture as well as mice lacking one of these genes, YY1, they found that, indeed, the two proteins did help move Xist back to the inactive X chromosome in activated lymphocytes.

They then conducted biochemical analyses to identify neuroligin - 3, confirm that the protein could stimulate tumor growth in cultured samples of several kinds of human high - grade gliomas and study which signals the protein uses within glioma cells to promote their growth.

Because hers were the first human cells cultured continuously for use in research, Lacks» identity was revealed in a scientific journal in 1971 in reference to the landmark accomplishment.

«Our group pioneered the development of cell culture technology for harvesting large numbers of stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that stem cells from these two sources are abundant and can be guided into different types of cells using tissue engineering.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

Investigations into human brain development using human cells in the culture dish have so far been very limited: the cells in the dish grow flat, so they do not display any three - dimensional structure.

Using cultured cells derived from human tumors of the breast and prostate gland, they confirmed that the IL6R / STAT3 / miR -34 a feedback loop is also activated in other tumor types.

At the time, his varied interests — in the use of skin cell culture to treat burns, in human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.

Surgeons report success in first human bone growth procedure using fat stem cells — with no culturing necessary

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Researchers have discovered a gene in zebrafish so powerful it can be used to redirect the fate of cells in the developing embryo to become beating heart cells, suggesting that a similar gene in humans could be used to generate heart cells in culture for transplant in ailing people.

Once primary cultures were established, the cells were immortalized with human papilloma virus E6 and E7 oncogenes using the disabled recombinant retroviral vector pLXSN16E6 / E7 (26).

Using the humble spinach leaf, the team successfully cultured beating human heart cells onto a leaf that's been stripped of its own plant cells.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Their results, published in Emerging Infectious Diseases, demonstrate that the highly differentiated 3 - D cell culture model can support the natural growth of human noroviruses, whereas previous attempts using differentiated monolayer cultures failed.

Three - dimensional neuroepithelial culture from human embryonic stem cells and its use for quantitative conversion to retinal pigment epithelium.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

Gage's team used human pluripotent stem cells to develop brain organoids, which were grown in culture for 40 to 50 days.

Human ES and iPS cells grow as monolayers in a completely defined and xeno - free cell culture environment that enables self - renewal and pluripotency without the use of ROCK inhibitors.

We use 3D stem cell («organoid») cultures from mice and humans to examine occurrence, level and molecular causes of CIN in cancer (Video 2).

The study used three cell types: human motor neurons derived from induced pluripotent stem cells, rat cortical cultures, and human breast cancer cells.

Human amniotic membrane (AM), the innermost layer of the placenta is the most common substrate used for both in vitro cultures and as a vehicle for transfer of cells during transplantation, due to its structural similarities to the ocular surface, as well as its biological and functional properties.

Human satellite cell cultures display numerous features of mature skeletal muscles (1) and have been used for studies of muscle metabolism in cultures established from patients with type 2 diabetes and healthy control subjects (1 — 5,8,9,21,22).

Dr. Masayo Yumoto, team leader of Regenerative Medicine Research & Planning Division, Rohto Pharmaceutical Co., Ltd., has been holding a leadership role in the joint research lab at the Institute of Medical Sciences, University of Tokyo, to develop next generation cell culture method using human mesenchymal stem cells.

To identify the JSRV receptor in lung cells, Miller developed a system using cultured hamster cells that contain small pieces of human DNA.

In light of the observation that iPS cell derivation takes place under the same culture conditions used for ES cells [20], we hypothesized that these human feeder cells could offer a stable tool for defining molecular hallmarks during conversion of differentiated somatic cells to the pluripotent state.

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

The «heart - on - a-chip,» which builds off previous successful iCHIP research on the peripheral and central nervous systems, involves the use of human cardiac cells cultured for up to nine days on the engineered chip.

HepG2 cells in 100 mm cell culture dishes were transfected with pGL - MARE - Luc plasmid DNA along with plasmid carrying the human Bach1 gene or the empty vector pFLAG - CMV - 6c using Fugene6 (Promega).

For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.

Specifically, the Ignatius laboratory will study tumorigenic cell populations that self - renew and metastasize using a combination of zebrafish, murine xenograft and human cell culture systems.

Herein, we used cell culture models of human mDA neurons to investigate a potential role for miR - 181a in mDA neurons.

We use a combination of conditional mouse models, primary cell culture (human and mouse), live microscopy, highthroughput genomics and proteomics approaches, and basic molecular biology.

Using a mouse model for this disease, which in humans involves the destruction of white matter in the brain, a research team led by Albee Messing, director of the UW — Madison Waisman Center, found that a protein behind the symptoms of the disease, called GFAP, is broken down more rapidly in the body than researchers previously found in cell culture studies.

This study used a model cell culture system that represents what happens in an animal or human.